Effect Of Extract Of Schisandra Chinensis On Growth Performance And Antioxidation In Weaned Piglets

Effects of extract of Schisandra chinensis (SCE) on growth performance, serum biochemicalparmeters, antioxidation and intestinal mucosal morphology were investigated in the current study.And the antibacterial effect of SCE with different concentration against piglet pathogenic bacteriain vitro was studied. And an experiment was conducted with weaned piglets to investigate theinfluence of SCE on intestinal Lactobacillus and Escherichia coli.Experiment 1: Effects of extract of SCE on growth performance of weaned piglets wereinvestigated. Twenty four 28-day-old piglets consisting of equal numbers of male and female wereassigned to two dietary treatment of six replications with two piglets per pen at random. Treatmentone was the control diet, and treatment two was the diet with 0.15% extracts of SC. The pigletswere fed from 29-day to 49-day old. The results showed that little differences were found on dailygrowth, daily feed intake, feed efficiency (P＞0.05).Experiment 2: Effects of extract of SCE on blood biochemical parameters of weaned pigletswere investigated. Piglets were slaughtered after 12 feed withdrawal. Blood was sampled from theanterior vena cava on the penultimate day after a 12 h fasting period. Blood samples weretransferred to glass tube, centrifuged after 30 min for 15 min at 1500 and the serum was frozen at-80℃until analyzed. Experiment showed extract of SC reduced triglyceride (TG) content in blood,significantly (P＜0.05). Little differences were observed on Glucose (GLU),Urea Nitrogen(BUN),Aspartate Aminotransferase (AST/GOT),Alkaline Phosphatase (ALP),AlanineAminotransferase (ALT/GPT),Low Density Lipoprotein Cholesterol (LDL),High DensityLipoprotein Cholesterol (HDL),Creatinine (CRE) and Cholesterol (CHO) contents in blood (P＞0.05). However, a tendency to reduced blood concentration of AST, ALT, LDL and HDL fed SCcompared to the control, and increased HDL concentration were observed.Experiment 3: Effects of extract of SCE on antioxidation of weaned piglets were investigated.Serum was separated and tissues incIuding heart, liver, kidney, and spleen were collected forantioxidation. The results showed the diets supplemented with SCE significantly increasedsuperoxide dimutase (SOD) activity of heart, liver, kidney, and spleen and glutathione reductase(GR) activity in serum (P＜0.05). No differences were found in SOD activity of serum and in GRactivity of heart and liver among the treatment (P＞0.05). SCE significantly reducedmalondialdehyed (MDA) content in serum, heart, and liver (P＜0.05). No differences were found inMDA content of kidney and spleen among the treatments (P＞0.05). The diets supplemented withSCE significantly increased T-AOC level in serum and heart (P＜0.05). No differences were found in T-AOC level of liver among the treatments (P＞0.05).Experiment 4: The influence of SCE with different concentration was observed on the growthof pig pathogenic bacteria cultured in vitro by E-test. The experiment results demonstrated thatSCE significantly decrease E.coli count of cecal content of piglet (P＜0.05). No significantdifferences between groups fed with SCE and the control on lactobacillus count of piglets(P＞0.05).Experiment 5: Effects of extract of SCE on intestinal mucosal morphology of weaned pigletswere investigated. The experiment results demonstrated that supplementation with SCEsignificantly increased villus height at jejunum. No differences were found of villus height atduodenum, and ileum, and of crypt depth at duodenum, jejunum, and ileum. SCE significantlyincreased the villus height to crypt depth ratio at duodenum and jejunum. No differences werefound of the ratio at ileum.