The Optimization On Tissue Culture Of Paeonia Suffruticosa Andr

Traditional breeding methods of Tree peony have low coefficient and long reproduction cycle.Using tissue culture techniques can obtain many fine varieties in the short term. Application ofmolecular biology techniques such as gene engineering, genetic transformation methods canimprove directional properties and develope new varieties, which are indispensable forefficient organization of the tissue culture system. This regeneration system is of greatsignificance to the effective usement of tree peony resources, breeding of new varieties andgermplasm creation, and can provide theoretical basis and technological support for the healthydevelopment of tree peony industry.The sprouts of different tree peonies varieties are taken as the explants, and the changes ofsprouts germination, multiplication and the induced rooting under different plant growthregulators and different environment factors are discussed. The scale of the main factor validconcentration in the culture process and the connection between the time of obtaining, thequality change of hormone and plant growth regulators are analyzed. The quality of hormone,phenol and the activity of PPO in different sprouts during various periods are measured. Theconnection between the quality of phenol, the activity of PPO and browning is analyzed.According to the selection of disinfection, medium and culture condition, the methods ofdisinfection, the factors of multiplication and the scope of the main factor valid concentrationare studied. At last, the effective method against browning is selected.Feburary is the best time to obtain the buds, because during this time the buds are sproutingand have higher hormone levels. The sprouting and multiplicating are also higher aftervaccination. The change of dormanting sprouts' quality of hormone is not the law, as well asthe difference among varieties. The sprouts contain high level of ABA, which maybe thereason of the low rate of germination. Compared with other hormones, the quality of ZR ismuch lower. The appropriate disinfecting time is 7-9min if use 0.1% HgCl₂, but if use Shang Nong todisinfect the explant by two steps, the disinfection is completely and no hurt to the explants.This management can simplify disinfection process and so can be used widely in peony tissueculture. The degree and number of browning are lower of those peony varieties which containlower quality of phenol and the activity of PPO, such as Hu Hong.The buds of Paeonia suffruticosa cv Hu Hong, Hei Hua Kui, Guan Shi Mo Yu, QingLong Wo Mo Chi, Lu Xiang Qiu, Dou Lu are induced in the test. The results states that thedifferences are very clear, Qing Long Wo Mo Chi is best on the rate of sprouts induced andmultiplication and Dou Luis the lowest. Guan Shi Mo Yu and Qing Long Wo Mo Chi arechoosen to compare the effect on multiplication under the different hormone (NAA & 6-BA)and the basic medium (1/2MS,MS,WPM) by L9 (3⁴) in the experiment. The result shows thatthe effect of the 6-BA is the most obvious, followed by medium and the effect of NAA;different hormone categories and concentration have the distinct effect. Adding 2-4mg/LAgNO₃ to the multiplication medium can improve the rate of multiplication. So the bestmultiplication mediums of Qing Long Wo Mo Chi and Guan Shi Mo Yu are MS+6-BA1.0mg/I+NAA0.4-0.6mg/L&MS+6-BA1.0mg/L+NAA0.4mg/L.During the course of rooting, medium types, and sugar concentration are the chief factorsabout rate of roots inducing. The IBA, sugar concentration and dark culture days have obviouseffect on the average length of roots. The medium of WPM+IBA6.0mg/1+Sugar 20g/L ishelpful to the roots inducing, WPM+IBA6.0mg/1+Sugar 40g/L and dark culture 12d arehelpful to the roots growth.