| Peony mature embryo , anther, sprout, immature embryo are taken as the explants in this paper. Complete multiple shoots regeneration system was established by the way of callus induction. Callus was obtained successfully by the way of anther ,and the anther was pretreated . According to the selection of disinfection ,the effective method against contamination was obtained.The induced rooting of sprout under some enviroment factors and plant growth regulators were researched,and the induced rooting of sprout complete system was established. Some culture factors under seedling formation of immature embryo 'Luo Yang Hong' were discussed,the appropriate medium type and mixture for tissue culture seedling domestication were selected.The main results were as follows:1.500 mg?L-1GA3 soaking treatment 48h was the best effective treatment in the aspect of breaking dormancy. 1/2MS+Ca2+ was the most appropriate basic medium in the culture of peony embryo. When the medium contain 2,4-D 4.0 mg?L-1+6-BA0.1mg.L-1callus was introduced well. The most optimum 6-BA concentration for introduction of multiple shoot from callus was 1.0 mg·L-1. The most optimum medium for plantlet regeneration was 1/2MS+IBA 1.5 mg·L-1+NAA0.5mg·L-1.2. The average frequencies of callus induction in the control group was obviously higher than cold pretreatment group and high temperature pretreatment group (P<0.05).The best medium of multiplication was MS+2,4-D 2.0 mg?L-1+6-BA1.0 mg?L-1, which average frequencies of callus induction reached 36.87%.3. The appropriate disinfecting time for sprout is 6-8min if use 0.2%(w/v)HgCl2.During the course of initial culture,first, sprout was cultured under dark for 7d,and then sprout was cultured under normal light condition,and browning was relieved obviously by the. way. The most optimum medium for root primordium was:1/2MS+NAA 2.0 mg·L-1+IAA2.0mg?L-1+6-BA 0.1mg·L-1orl/2MS+NAA1.0mg·L-1+IBA1.0mg?L-1+AC0.2g?L-1.4. The best medium for seedling formation of peony 'Luo Yang Hong' was: 1/2 MS+6-BA 0.1mg·L-1+IBA 0.8 mg·L-1+LH 100 mg·L-1. Appropriate matrix for domestication was humus soil:'Luo Yang' soil=1:1. |
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