The Establishing Of Micropropagation System Of Malania Oleifera And Study On The Technique Of Cell Suspension Culture

The thesis was completely analyzed in the micropropagation and cellsuspension culture technology of Malania oleifera. The micropropagation systemof Malania oleifera was set up and the suspension culture condition of Malaniaoleifera was improved effectively. This research results will provide someresearch information in the micropropagation of Malania oleifera and extractingthe effective ingredients of Malania oleifera by cell suspension culturetechnology.1. The combination of 70%ETHANOL 15s+10%NaC10 12min+0.1%HgCl₂ 8min was mostefficient for stem sterilization of the explants, and 70% ETHANOL 30s+12%NaC1010min+0.1%HgCl₂30min had the best sterilizing effect for seeds.2. The experimental results of 5 basic mediums which included MS, 1/2MS, B5, Whiteand Miller showed that MS medium was suitable for asepsis seedling inductionof seed embryos of Malania oleifera.3. The pathway of adventitious bud differentiation may become the main pathwayon tissue culture to attain the tube plantlets.4. GA₃ can facilitate the bud multiplication growth in the subculture. 5. The optimun medium for callus induction of hypocotyledonary axis of asepsisseedling was MS+2, 4-D 1.5 mg·L^-1+6-BA0.6 mg·L^-1+NAA1.0mg·L^-1, and the inductionrate was 100%.6. Sand was the best matrix for transplant the plantlets of Malania oleiferaand the rate of survival was 86.67% in 40d.7. The optimal medium for the callus induction of endosperm was MS medium with2,4-D2.0mg·L^-1+KT 0.2mg·L^-1+6-BA0.4mg·L^-1+GA₃0.4mg·L^-1,and the inductionrate was 100%.8. The optimal medium for the callus multiplication of endosperm was MS mediawith 2,4-D 1.0 mg·L^-1+6-BA0.3 mg·L^-1+GA₃0.3 mg·L^-1,and the multiplicationmultiple was 2.17.9. The first two generations of calli had the highest ability of multiplictionand differentiation, and the multiplication multiple was over 2 folds.10. The most optimun culture conditions for suspension culture of endospermcallus of Malania oleifera showed as following: Using MS as basic medium withsucrose as carbon source that the concentration was 40g·L^-1, the optimun pHwas 6.0 and the agitation rate was 110r/min. After culturing for 20 days, theyield of 15c-tetracosenoic acid in the medium was 2.9g·L^-1.