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Adsorptive Behavior Of Viruses To Soils And Its Significance In The Environment

Posted on:2007-04-04Degree:MasterType:Thesis
Country:ChinaCandidate:Q Y WangFull Text:PDF
GTID:2143360212955074Subject:Soil science
Abstract/Summary:PDF Full Text Request
Pathogens such as bacteria and viruses are widely spread in septic tanks, sewage sludge, wastewater and other sources. Groundwater contamination with viruses has caused great concerns in various countries because viruses are much smaller in size than bacteria or protozoan cysts and not easy to be filtered out through the porous soil matrix. Bacteriophages MS2 and φX174 have been used e as indicator viruses in this paper and have been used to study virus adsorption in the lab, providding scientific thereunde for the sake of evaluate the action ,which filtrate and decontaminate viruses ,of different soil with different disposal.Requirements and general procedures for the laboratory propagation of bacteriophages MS2 and φX174 by double layer agar plate with small amount of phage harvesting and high multiplicity of infection with large amount of phage harvesting, and extracte viruses from the sample in the encironment and food, were explained in detail. Batch sorption experiments were carried out with the bacteriophages MS2 and φX174 to compare different adsorptive behavior of viruses to 6 different soils with contrast properties. The effect of the presence of indigenous microorganisms on the adsorptive behavior was also studied.And compare different survival of viruses to 2 different soils with different properties and moisture. The effect of the presence of indigenous microorganisms on the survival was also...
Keywords/Search Tags:Bacteriophages, MS2, φX174, Requirements propagation, virus adsorption, Virus survival
PDF Full Text Request
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