Study On Diagnosis By Reverse Transcription Polymerase Chain Reaction And Antibody Detection Of Hog Cholera

Hog cholera that is aroused by hog cholera virus is a kind of high contagious and infectious disease with high death rate all over the world. It is a great danger to pig cultivation industry. Many countries have drawn up veterinarian's laws and regulations, wholly enter in a list as hog cholera. One of legal infectious disease, and in immediate future have to list what appears in the newspaper the epidemic disease. Healthy Statue Book Of International Animal that international veterinarian of French Paris innings draw up in case being living. The hog cholera is entered in a list one of 16 kinds of legal infectious diseases of A degree, and requires the belonging to the nation obligation speech epidemic situation of every member, and according to the stipulation quarantines with sticks to the relevant stipulation that the internation intercharged Being living our country in Domestic Animal Poultuy Epidemic Prevention Rules Implementation Manuals, the hog cholera is too entered in a list as One type of infectious disease.In the recent years, with the wake of raising pigs, the swift development of trade was raised pigs to especially scaleization in closer year, and the distinguishing feature happened the very great altetion to hog cholera prevalent and suffers from an illness, and such prevalent condition transforms to little scale cyclicity with the huge current through frequently happen is competent, and wave style area is prevalent as sending forth quality. Suffering from an illness, the distinguishing feature is give priority to with non-type's hog cholera. The diagnosis to native illness of diversified quality of hog cholera conduct brought the greater hardship, and easily causes making a wrong diagnosis. Virus in common use of the laboratory technique step such as departs and immunity diagnosis and so on is with the necessatise that can not meet the fresh condition. Hence launches to research saving to establish one kind of more well-developed, more valid diagnosis technique, along with popularizing and applying, and havees the significant practice sense to precaution and control hog cholera.As a reference goal the article is original, which is consulted and follow with hog cholera virus E2's gene set issueing, and the design was synthetised one pair enlarging that distinctive quality that gains the pig or small 443bp of passage stretches the matter. In the way of RT-PCR's means adjust the powerful toxin of hog cholera criterion stem, the feeble toxin of hog cholera rabbitization stem, 9 suspects that the hog cholera is anticipateed along with the comparison series BVDV, foot-and-mouth disease virus (FMDV's vaccine). Examine the diagnosis, kill this one to stretch the matter and adjust the powerful toxin of hog cholera criterion stem, the feeble toxin of rabbitization stem and in it, four of them are suspected that the illness all anticipates that distinctive quality enlarges to gain RT-PCR's result is agaist 443bp who anticipates the size to tally with. But comtrasting else swines, the person who falls ill enlarge gain the result act as the feminine. The hog cholera is suspected to be similar the illness and is anticipated after the rabbit part of the body mutually that the immunity is tested, and kills that 4 of the masculine of RT-PCR suspects to be similar the illness and all anticipates to be decided that to have swine fever virus, and that the experiment kills to the feminine is anticipateed that to the reat illness.Making known RT-PCR is one kind of distinctive susceptible means to the hog cholera diagnosis, is living that I save and first establish the diagnosis means of hog cholera RT-PCR. It is the main measure that our country provided prevention and cure swine fever at the moment that the vaccine has an inoculatiom, and such essence is hog cholera antibody which surely measured by coming into being inner place the swine part of the body, and what it is in order to stand up to hog cholera virus invade and attack, there by attains the prevention and cure hog cholera aim. As a result the hog cholera antibody monitoring how adjust the immunity, and causes the swine come into being hog cholera antibody in good time, and havees the extremdy significant action. The article was from indirect hemagglutination test. 105 piglets from healthy sows of Chuxiong Pig Farm of Yunnan Province were selected for forwand indirect hemagglutination test. And these piglets were inoculated with hog cholera virus before suckling whereas others are selected as comtral for antibody detect regularly. Characteristic of the immune and maternal antibody's change are understood by us, which set a base for our hog cholera inoculating plan, the effective actions are based on all of them.