Screening And Identification Of Variations Of Soybean Seed Storage Protein Subunits And Their Genetics Stability And Functional Properties

The contents of 7S and 11S globulins and their respective subunits, and the 11S/7S ratios in seeds of 1400 soybean varieties were analyzed using SDS-PAGE, the affection of varieties and years on the major fractions and their subunits, further identification the kind of subunits deficiency, the genetic analysis of A_1aB_1b-subunit and A₃B₄-subunit in soybean seeds, integration null mutations for A_1aB_1b-subunit, A₃B₄-subunit and A₅A₄B₃-subunit of the storage protein were by crossbreeding, induced variation germplasm of storage proteins by physical and chemical method, and the relationships between subunits variation and functional properties of soybean protein were analyzed. The main results are as follows:1. The average and variation range of the 11S/7S ratios of 1400 soybean varieties were 1.68±0.37 and 0.44-3.64, and 34 variation accessions of subunits content were identified, including low level of α'-,α-subunit, low level of β-subunit, absence of A₃B₄-subunit, absence of A₅A₄B₃-subunit and A_1aB_1b-subunit. The results of induced variation germplasm of storage proteins by physical and chemical method indicated: the chemical induced rate is 2. 92%, while the physical induced rate is 5.71%, ⁶⁰Co γ-ray irradiation treatment > 0.4%EMS treatment > 0.2% EMS treatment, and 211 seeds of subunits variation were detected in the M₃ seed.2. The content of 11S and 7S fractions and their major subunits in 280 soybean varieties storage protein during 2003, 2004 and 2005 years were compared with SDS-PAGE. The results showed that there were significant differences on the content of α', α, β, A₃, A₄, A₂A_1aA_1b, B₃, B_1aB_1bB₂, B₄, A₅ subunits, 11S and 7S, 11S +7S and 11S/7S between difference varieties, while except A₄-subunit there no significant differences among 2003, 2004 and 2005 years.3. Basis on successfully selected a mutant line which characterized by the lack of the A_1aB_1b or A_1bB₂ or A₂B_1a -subunits of glycinin from cultivar variety "Guiyangzijindou", the proteome of the seed protein of soybean varieties "Nannongdahuangdou" and...