Studies On Inheritance Of Male Fertility Restoration And SSR Markers Location Of Restorer Gene Of Cytoplasmic-Nuclear Male-Sterile Line NJCMS1A In Soybeans (Glycine Max (L.) Merrill)

The utilization of the heterosis is one of the important ways to increase the production of soybean greatly. At present, tens of different cytoplasmic-nuclear male-sterile lines with stable male sterility have been developed in soybeans. Their maintainer lines and restorer lines also have been obtained, which make it possible to utilize soybean heterosis. The inheritance of the male fertility restoration of the cytoplasmic-nuclear male-sterile line of soybean is the basis of the utilization of the heterosis. In this paper, the inheritance of the male fertility restoration of the cytoplasmic-nuclear male-sterile line NJCMS1A of soybean was studied, and the SSR markers linked to the restorer gene of NJCMS1A were also screened. The main results were as follows:1.The male sterility of NJCMS1A was confirmed high (the germination percentage of pollens was 0.00%) and quite stable. The results of the performance investigation of the male-sterile plants in mature period were in agreement with those of the investigation of anther dispersion and the investigation of the germination percentage of pollens. Therefore, all of the three methods could be used to distinguish the male-sterile plants from the male-fertile ones. Among the three methods, the identification of the male-sterile plants according to the anther dispersion was simple and easy to operate, and could be used to identify the male-sterile plants at the early stage of the growth of NJCMS1A plants in large production and hybridization.2. Four soybean materials were crossed with NJCMS1A. The fertility analysis of the F1 plants showed that Zhongdou5 was a very good restorer line of NJCMS1A, Nannong 94-16, Nannong 94-156 and Youchu 4 were the incomplete restore lines of NJCMS1A. So the combination of (NJCMSlAxZhongdou5) was chosen to study the inheritance of the male fertility restoration of NJCMS1A and to screen the SSR markers linked to to the restorer gene of NJCMS1A.3. The ratio of fertile plants and sterile plants of F2 population of the combination (NJCMSlAxZhongdou5) was demonstrated to be 3:1, either inspring or in summer. It was showed that the ratio of fertile plants and sterile plants of F2 population of the combination (NJCMSlAxZhongdou5) wasn't tampered with the circumstance factors such as sunlight, illumination, temperature etc. The inheritance of the male fertility restoration of NJCMSIA was stable.4. For all the F1 plants were fertile, and the ratio of fertile plants and sterile plants of F2 population of the combination (NJCMSlAxZhongdou5) was tested to be 3:1, it was concluded that the male fertility restoration of NJCMSIA was controlled by one pair of dominant genes. The restorer gene is dominant, and the sterile gene is latent.5. 498 pairs of SSR primers of soybean were chosen to screen the polymorphic bands between NJCMSIA and Zhongdou5.111 pairs of SSR primers were found to be polymorphic between NJCMSIA and Zhongdou5. Then these 111 pairs of SSR primers were used to map the restorer gene through F2 population. The results showed that the restorer gene of NJCMSIA was linked to such SSR markers as Sat-036 and Satt 179 on D1a+Q chromosome, and Sat-141 on D1d+W chromosome, and Sattl45 on F chromosome. The recombinant value between the restorer gene of NJCMSIA and Satt179, Sat-036, Sat-141, satt145 was 17.8cM, 21.0cM, 24.3cM, and 29.8cM respectively.