| The new preparations of hypericin was developed successfully in LanZhou Institute of Animal and Veterinary Pharmaceutics Science of Chinese Academy of Agricultural Sciences for the first time. It was mainly used to inhibiting animal disease such as anti-virosis. The most important component of the new preparations was hypericin, which was extracted from hypericum perforatum Linn and had effect on anti-virosis of animals.Acute and subacute toxicity experiments of the new preparations of hypericin were proceeded to evaluate the security of the preparations objectively so that the new preparations was used in clinical widely. First,30 mice were divided into six pharmaceutical dose groups (0.8g/kg,1.3g /kg,2.2g/kg,3.6g /kg,6.0g/kg and 10.0g/kg). According to these datas, preparing acute toxicity experiment was proceeded. It was concluded that the toxicological phenomenas won`t observed in six giving pharmaceutical dose groups. The results showed that the random of dose of this experiment was narrow and needed to improve dose random in formal acute experiment. Second,50 mice were divided into five pharmaceutical dose groups on the basis of preparing experiment(7.5g/kg,10.0g /kg,13.3g/kg,17.7g /kg and 23.6g/kg). According to these datas, the formal acute toxicity experiment was proceeded. It was concluded that these mice of five giving pharmaceutical dose groups didn't appear toxicological symptoms, and that the clinical representation and histology checking had no abnormal surroundings. The results showed that the toxicity of the new preparations of hypericin was very low. Because of the acute toxicity experiment in mice didn't give the LD50 of the new preparations of hypericin,acute toxicity experiment in chicken was proceeded,50 chickens were divided into five dose groups(13.3g/kg,17.7g /kg,23.6g/kg,31.5g /kg and 42.0g/kg). It can be drawn conclusion that the LD50 of the new preparations of hypericin was 32.42±0.0312g/kg.The results showed that the new preparations of hypericin was a practical non-poisonous drug.According to standard, subacute toxicity experiment was proceeded necessarily to evaluate the security of the new preparations of hypericin. So subacute experiments of chicken and rat were proceeded. There were three giving pharmaceutical dose groups of subacute toxicity experiment in chikens, the high dose group was 3.24g/kg,medium dose group was 0.76g/kg and the low dose group was 0.22g/kg. The three giving pharmaceutical dose groups of rats were 2.95g/kg,0.76g/kg and 0.20g/kg. In these experiments, all the animals were given... |
