Study About Acute, Subacute And Cumulative Toxicity Of Sulfaquinoxaline On Mice

In this study, according to the first and second stages of the content of Procedures for Toxicological Assessment of Food (GB15193.1-2003). Mice were used applied as experimental animals, and Sulfaquinoxaline(SQ) poisoning model was replicated, toxic symptoms, hematology parameters,changes of immune function and pathology were observed. For a more systematic study, the dose-effect relationship, the degree of accumulation and toxicity hazards were included in order to provide a theoretical reference for the production of pollution-free meat and to provide a strong basis for monitoring strictly on the quality and safety of livestock products. Acute toxicity—LD50 of SQ to mice was determined by modified Karber method. In subacute toxicity test,40 mice were randomly allocated into 4 groups with 10 each, half male and half female. GroupⅠ～Ⅲwere treated as experimental groups, while group IV as control. GroupⅠ～Ⅲwere gavaged with SQ solution 150mg/kg,75 mg/kg and 37.5mg/kg respectively, and groupⅣwere gavaged with the same dose of distilled water as control. Daily administration for 30 days.In accumulation of toxic test, detected Sulfaquinoxaline accumulation factor in mice. Clinic symptoms were observed after mice were gavaged with different doses of SQ solutions.6 hours after the last administration, enucleation method blood, and detect hematology. Then mice were killed, and computed the organ indices. Main organs were detected, for the organs with obvious pathological changes, paraffin sections observations were performed. The results showed that LD50 to mice by oral administration was 3138.34mg/kg, and 95% confidence limit was 2755.83-3573.95mg/kg. The orally accumulation coefficient K of Sulfaquinoxaline in mice is 3.3, medium accumulation. As dose increased and time went by, poisoning symptoms were obviously aggravated. The number of WBC and lymphocytes of experimental mice reduced, BUN and Cre levels increased, ALT and AST activity increased. The liver index and kidney index increased as the dose increased, which means functions of livers and kidneys were damaged. The spleen index, thymus index and heart index were decreased as dose increased, which means immune function of mice were inhibited, and heart function were harmed in certain extent. Livers and kidneys were obviously enlarged, colors of spleen were deeper slightly and pericardial fluid increased in pathological anatomy. Myocardial fiber were swelled and degenerated, splenic sinus congested and dilated, giant corpuscle increased, capillary swelled, cellula epithelialis of nephric tubule in kidney cortical area degenerated, capsula glomeruli expanded and glomerulus shrinked, a large of lightly stained material leaked to renal capsules and deposited in pathology histological observation.In summary, SQ damaged heart, liver, spleen, lung, kidney and other organs of mice with obvious clinic symptoms, immune activity dropping, and obvious pathological changes which depended on dose-reaction, multiple doses will accumulate in the body.