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Studies Of Application On The Program Artificial Insemination In Mutton Sheep

Posted on:2006-03-11Degree:MasterType:Thesis
Country:ChinaCandidate:D P BaiFull Text:PDF
GTID:2143360185490161Subject:Animal breeding and genetics and breeding
Abstract/Summary:PDF Full Text Request
In this study, the program artificial insemination of mutton sheep in was established through estrous synchronization, chilled storage semen, semen optimal dilution bind with spermatozoa number. The results were as follows.Four estrus synchrony scheme of PG (0.1 mg) + Norgestrel-sponge (60mg) + FSH (40IU), PG (0.1 mg) + Norgestrel-sponge (60mg) + FSH (60IU), Norgestrel-sponge (60mg) + FSH (40IU), Norgestrel-sponge (60mg) + FSH (60IU) were used for Han sheep ewes induced estrus synchronization. The results of four estrus synchrony schemes were satisfied in this experiment, estrus synchrony rates were 96.5%, 94.7%, 90.0%, 92.9%(P>0.05), respectively. Norgestrel-sponge (60mg) +FSH (40IU) was more economical and effective than other three schemes for the induction of synchronized estrus in sheep breeding and reproduction.Optimal extenders of five were selected to dilution and chilled storage of ram semen through observation of general survival time, available survival time, survival index of sperm. The results showed: 4 times dilution was not suited for semen storage, 8, 12, and 16 times dilution were significant better than 4 times (p<0.01), in view of ewe's conception, 8 times dilution was better than 12 and 16 times in storage semen;The extender based on glucose 3.0g, citrate sodium 2.0g, yolk 20ml was better for chilled storage than other extenders (p<0.05), and selected for ram semen chilled storage.Inseminated with numbers of ram's spermatozoa were 20 million, 30 million, 40 million, 50 million, 60 million, 70 million, and 120 million on seven treatment group (355 ewes), to select the optimal spermatozoa number through 25d not return estrus after AI. The 25d NR rate were 43.24%, 42.22%, 50%, 60.47%, 59.62%, 62.79%, 62.63% in seven treatment group, respectively. This result showed that 25d NR rate was enhanced with numbers of spermatozoa increased when spermatozoa under 50 million, the 25d NR rate is positive correlation with the spermatozoa number(R=0.9402). With a view of ewe's conception rate and increasing ram's use ratio, the numbers of spermatozoa is not low 50 million in sheep inseminated.The program artificial insemination systems of mutton sheep were established according to principle of scientific, simple, and easily operate. The systems were composed of semen-supplied station, semen-supplied spot, and ewe's AI spot. Predict the profit of...
Keywords/Search Tags:Sheep, Estrus synchrony, Chilled storage ram semen, Semen dilution, Program AI
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