| Rabies is a drastic infection disease caused by rabies viruses and rabies relative viruses. Almost all the homeothermal animals can infect rabies virus, and the mortality rate is 100% once they infect rabies virus, there are 55000 persons died by infection of rabies virus. In recent years along with molecular biology development and application, almost aspects of rabies virus are known, such as virus structure, the gene sequence, the main function and so on. In order to rich rabies virus gene bank, sequencing and analyzing parts of the two rabies virus street strains isolated from different animals in China.In this study, the cDNA fragments of G-L intergenic region(IGR) and polymerase activity module in L gene of two street rabies virus were cloned and sequenced. The nucleotides sequence of these cDNA fragments mentioned above and their deduced amino acid sequence were compared with that of rabies virus strains, rabies-related virus strains and other Rliabdoviridae published previously. The result show that the homology of nucleotide sequence of G-L IGR among MRV, DRV is 83.3%, compared with other rabies-related virus strains, the homology of G-L IGR is between 58.3%-91.7%. the length of G-L IGR of rabies virus is different in Rhabdoviridae. It can be divided into two species according to weather there are inserted genes in G-L IGR. The one has no inserted genes between G-L gene including Lyssavirus and Vesiculovirus, the other has one to six inserted genes between G and L gene including Ephemerovirus and Novirhabdovirus. It is speculated that this may be the result of fitting the inquilinous tissue cell during evolution of Rhabdoviridae.The homology of nucleotide of polymerase activity in L gene between MRV and DRV is 100%, compared of the sequences of nucleotide and amino acid of polymerase activity module of MRV and DRV strain with that of other fixed rabies virus strains and rabies-related virus strains, the nucleotide homology between MRV and DRV is 100%, and that of strains in MRV, DRV and other fixed viruses were 83.2%-100%, their homologies of deduced amino acid were ranged from 83.8% to 93.8%. In contract with that of fixed strains, the amino acid on position 672, 681 and 745 of L protein in MRV, DRV were different, polymerase activity module of L gene is comparatively conserved, thus it is propitious to the stabilization of polymerase activity. |
