| G gene of rabies virus M, The of two main regions (about 1000nt), ranging from 3161nt to 4162nt and ranging from 4012nt to 4863nt of glycoprotein gene of rabies virus strain M, isolated from mouse in He Nan, China were amplified by reverse transcriptase-polynerase chain reaction (RT-PCR) in order to complete glycoprotein gene of strain M . These regions were sequenced by the produce of PCR directly. Comparison and analysis of nucleotide sequence and amino acid sequence deduced with that of other strains published was performed by computer with DNAsisv 2.5Demo software. The result showed that the homology of strain MRV, CVS (challenge virus standard) is the highest (96.5%) in the same genotype. On the contrary, the homology with strain CTN is the lowest (79.8%).In addition, the result proved that among the four functional regions of glycoprotein, the homology of amino acid sequence is the highest in the Antigen regions, instead of the lowest in cytoplasmic domain. And amino acids of strain MRV between Antigenic site and glycosylation site is different. To some extent, it will decide and change the antigen of MRV.Phylogenitic tree was established by analysis and comparison. Strain MRV and strain CVS are belong to the same genotype. However, strain MRV, CTN and CNX8511 are belong to different sub-genotype. Finally, this researched also proved that the character of neuron tropism rabies viruses by comparison and analysis the homologies of amino acid sequence between 189-214 position and the bonding segment of snake venom curaremimetic neurotoxims and acetylcholine. |
