The Clinic Pharmacodynamics Appreciation And Development Of Compound Preparation "Runaikang"

The mastitis of dairy cow was one of the most frequent diseases in the breeding industry, which often brought great detriment to the industry. In present, researchers actively searched one effective method to control the disease all over the world, but a significantly effective method which could prevent and cure the disease was not existed, owing to the complexity of the pathogenic microorganism which could cause the mastitis.According to the pathogenesis of dairy cow mastitis, pharmacology and pharmaceutics, we investigated the pathogenic bacteria of the mastitis in Taizhou city of Jiangsu province. And some experiments were carried out, for instance, the isolation of the main pathogenic bacteria, pungent test, drug sensitive test and drug residue. To develop a complex antibiotic prescription this would be economical, applicable, safe and high-performance.The results were showed as following.(1) The incidence rate of the recessive mastitis was 58.8%, while the incidence rate of the clinical mastitis was 3.81%. The isolating rate of the staphylococcus aureus, the staphylococcus epidermidis and the staphylococcus saprophyticus was 42.31%, 23.08% and 6.41%, respectively. However, the isolating rate of the streptococcus dysgalactiae, the streptococcus uberis and the streptococcus agalactiae was 8.97%, 5.13% and 1.28%, respectively. The isolating rate of the escherichia coli of Enterobacteriaceae was 6.41%, and the isolating rate of the yeast of fungi was 2.56%, the other three bacterium were isolated and the proportion of them was 3.85%.(2) The results indicated that the ceftriaxone sodium and amphenicols had the better antiblastic effect on the pathogenic bacterium of the staphylococcus. Quinolones, amphenicols and bactrim had the better antiblastic effect on the pathogenic bacterium of the streptococcus. And the ceftriaxone sodium and quinolones had the better antiblastic effect on the E.coli. MIC and MBC of RuNaikang in the isolated pathogenic strain was 1487.777μg/ml and 1888.887μg/ml, respectively.(3) The RuNaikang had a slight hormesis to the tissue of mammary gland, but no obvious damnification occured. The stimulatory function of RuNaikang was similar to the Sodium Chloride.(4) The residue level of effective constituent of RuNaikang in the milk during the different period was measured by the HPLC method, and the result showed that the residue could not detected in the milk at 3.5 day following drug withdrawal, meeting the encoding standard which should lower than 3ug/kg. Therefore, the withdrawal period of the milk could be determined as 3.5 day. (5) The test of clinical pharmacodynamics indicated that RuNaikang had better treatment effectiveness, which had corresponding effect compared with the import Sunuo. So RuNaikang should be a therapeutic drug which would be economical, applicable, safe and high-performance.