Medium Selection And Genetic Diversity Of Pleurotus Eryngii And Preliminary Assession Of ITS And LSU Partial Sequences As DNA Barcoding Genes

Pleurotus eryngii is a widely distributed wood-rotting fungus with long cultivation history. At present, cotton seed shell, sawdust, corn cob and wheat were used to cultivate Pleurotus eryngii. Screening medium and cultivation of fruit body tests were provided in this paper. The best substrate for mushroom growth, improve the quality and yield of culture medium were selected. Peanut hull was one of the agricultural wastes and was used to make mother media and culture media. The effect of it on the growth of Pleurotus eryngii mycelia was tested in this study. The results showed that the mycelia growth rate on the medium with peanut hull was higher than other substrates. The best ratio was peanut hull:potato=3:1, on which the mycelia grew fast to 5.43 mm/d. The B substrate was determined to be suitable for the fruit body, which mycelia grew fastest (5.43mm/d), vigorous, white and intensive on yield per bag reached highest (584.3g), while the cultivation time was short(22.9d).This test used a new method of molecular marker-SRAP (sequence related amplified polymorphism). Nineteen different sources of genetic diversity of strains of Pleurotus were used to discuss the distance of the genetic relationship. The results showed that:SRAP markers divided those 19 species into three categories at the level of 87%. The first branch was composed of No.51338 strain, which was brought from China Agricultural Culture Collection Centre, ped, gaorong, rixing, xinghan, wuxing1, wuxing2, shennong, xing3, xing1,50961 and yannong were the second largest category. This branch indicated that their genetic distance was close. Seven strains (ped6320, xing910, yurun, chaoxing,51678,50931 and xing2) are the third largest category. This study indicated that most of the domestic mushroom strains were close related and shared a common ancestor.ITS and LSU region of Pleurotus eryngii, Pleurotus nebrodensis, Pleurotus ostreatus, Pleurotus citrinopileatus, Pleurotus pulmonarius, Pleurotus djamor, Pleurotus cornucopiae, Pleurotus abalonus, were amplified by using their cultivated material. Phylogenetic analysis of eight kinds of relationships, resulting in a species can be identified genes. The results show that eight species of fungi Pleurotus gene in the ITS phylogenetic tree, the branch most capable of forming an independent. Only the Pleurotus eryngii, Pleurotus nebrodensis were downloaded from GenBank. The LSU gene sequence with the same number of species (Pleurotus ostreatus, Pleurotus pulmonarius, Pleurotus cornucopiae), suggesting that the LSU gene was unfit for species level identification. LSU and ITS sequences were more suitable for species identification and it can be considered as a bar code label for the future to provide a theoretical basis for selection.