Researches On Constituents Of Herba Scutellaria Barbata In Anticancer

Herba scutellariae barbatae is the dried whole plant of Scutellaria barbata D. Don which belongs to Scutellaria Labiatae, distributed in the east, south, southwest China and Hebei, Henan, southern Shaanxi, Hubei, Hunan and other places. Herba scutellariae barbatae is spicy, bitter in flavor and cold in property, and it is attributive to the lung meridian, live meridian and kidney meridian. It's a commonly used traditional Chinese medicinal material in prescription to clear away heat and toxic materials, resolve stasis and diuretic, and used for swollen boils, sore throat, snake bites, flutter swelling, edema, and jaundice.Cancer, also known as malignant tumors, is one of the major diseases harming human health. The anti-tumor active ingredients extracted from traditional Chinese medicinal materials have been paid more and more attentions in recent years. Herba scutellaria barbatae has been one of the main focus in researches on anticancer drugs at home and abroad in recent years. Modern pharmacological studies have shown that the extracts from herba scutellaria barbatae by water and ethanol have significant anti-tumor effects in different mechanisms, including inhibiting tumor growth and proliferation directly, inducing tumor cell apoptosis, antioxidation and clearing free radicals. There are few published papers about antiangiogenic activity of herba scutellariae barbatae and its chemical compositions with a few in vitro tests and no papers about studies on antiangiogenesis of it and its extracts on basis of live animals. Screening active compounds from herba scutellariae barbatae by using zebrafish antiangiogenesis model in this paper hasn't been reported currently.In this paper, systematic studies on 75% ethanol extracts has been finished. Firstly, three parts with different polarities were got by solvent extraction, which are petroleum ether extract, ethyl acetate extract and butanol extract. After screening with zebrafish model, the ethyl acetate extract was determined to have antiangiogenic activity. Secondly, 13 compounds were got from ethyl acetate extract by silica gel column chromatography, gel column chromatography, recrystallization and etc. These compounds were determined to beβ-sitosterol, wogonin, 4'-hydroxy wogonin, apigenin, baicalein, naringenin, luteolin, isorhamnetin, quercetin,β-daucosterol, wild baicalin, baicalin and luteolin by means of NMR and physical and chemical properties analysis. Lastly, we used the zebrafish model to envaluate the antiangiogenic activity of these 13 compounds, and the results show that quercetin and isorhamnetin have significant antiangiogenic activities with inhibitory rate of 76.79% and 61.24% at 100μg/mL, respectively.