Component Analysis Of Lycoris Radiata Alkaloids And The Initial Research Of Lycoris Radiata Alkaloids's Biosynthesis

In this study the tissue culture conditions of Lycoris radiata was investigated; the extracting conditions of galantamine in L. radiata was optimized; the TLC analysis method of alkaloid in L. radiata was constituted; the influence of galantamine, lycoramine, lycorine on the biosynthesis of L. radiata alkaloids was studied(liquid tissue culture method was used); and the changes of alkalods content in the process of bud induced tissue culture was studied through the solid culture, on the while, the influence of galantamine on the biosynthesis of L. radiata alkaloids was studied. The initial research was carried through on L. radiata alkaloids biosynthesis pathway. The main cotent as follows:The method of L. radiata fast breeding was erected.The result showed:the medium MS+0.3 mg/LNAA+3.0 mg/L6-BA was optimal for bud induced, MS+0.5 mg/L 6-BA +0.8 mg/L NAA was the suitable medium for root induced, the nutrient soil with 30% vermiculite made for the survie of young plants.November was the suitable growth period for the tissue culture of L. radiata bulbs.The study about the seasonal charactar of L. radiata tissue culture was the first time.Through the single-factor and orthogonal experiment the better test conditions was obtained, as follows:75% ethonal,microvave processing time 60 S,marinating time lh,solid to liquid ratio 1:10,microwave power 480 W. The galantamine cotent of different parts in L. radiata was studied by these conditions,the result was:the content in bulb was the most, footstalk, flower, leaf took turns.This method could extract more alkalods in short time,. was suitable for dealing with lots of materials whenthe biosynthesis pathway of L. radiata alkaloids was studied.In this experiment one system and two systems TLC analysis methods was studied. In one system TLC methods, solvent CHC13:MeOH:NH3.H2O(9:1:0.2)was the best for Lycoris radiata alkaloids separated, showed the most spots which were very clear.The effect of two systems methods was better than one system methods. Two system was solvent 1 EtOAc: MeOH:NH3.H2O(9:1:0.2), solvent 2 CHC13:MeOH:NH3-H2O(9:1:0.2). Which could separate more L. radiata alkaloids than one system method, and the spots were all clear-cut. It was a new method to separate L. radiata alkaloids, spent less materails and took little time, was very facility.Wafer of L. radiata bulbs was cultured in liquid MS medium,and the effect of galantamine, lycoramine, lycorine on the biosynthesis of L. radiata alkaloids was studied. The results showed that galantamine, lycoramine, lycorine could inhibit the biosynthesis of L. radiata alkaloids obviously. We could speculate that all Lycoris radiata alkaloids had one common precursor, and we presumed that were in the same way of Lycoris radiata alkaloids biosynthesis, alkaloid "d" and "e" were located between and lycoramine. Lycorine and alkaloid "I" were in the other embranchment.The alkaloid content changes was studied in bud induced tissue culture process. Except galantamine, all the other alkaloid content increased and then decreased, but galantamine had been increasing all the while.After galantamine added, we acquired the same result as the bulb wafer's liquid culture, galantamine inhibited the biosynthesis of all alkloid in L. radiata. This method also could study the biosynthesis of L. radiata alkaloids. It's another method to study L. radiata alkaloids biosynthesis.