Study On Physicochemical Properties And Related Pharmacological Effects Of Polysaccharides From White And

Bletilla (Bletilla striata (Thunb) Reichb.f.) which is a kind of rare orchid traditional Chinese herbal medicine. BSPS(Bletilla striata Polysaccharide, BSPS) is the main ingredient of Bletilla striata. Which are widely used for the treatment of viral, tumor, bleeding, peptic ulcer bleeding, regulating the immune functions, prompting would healing and so on. It shows great value and development prospect in medicine and functional food fields. However, the mechanism of BSPS is not well understood. In this study, BSPS, whose application prospect is extensive promote.1. Ethanol-alkali extraction, isolation, purification and analysis of BSPSIn this study, With Bletilla striata’s dry tuber as experimental materials. BSPS was extracted by the dilute alkaline solution. Then it was precipitated by of ethanol. Through a series of processes in turn, taking off the protein by repeating freezing and thawing and Sevag method. Removal oligosaccharides and small molecular by dialysising. Five purified polysaccharide fractions BSPS-Ⅰ～Ⅴ were successfully obtained after using separation column (DEAE-Sepharose Fast Flow).Analysising them physical properties. Molecular weight size (HPLC):355.8kDa,174.3kDa,2.8kDa,174.0kDa,59.9kDa. The mole ratio of monosaccharide chemical compositions (GC), BSPS-Ⅰ and BSPS-Ⅲ:Glu; BSPS-Ⅱ:Man:Glu=1.05:4.22, BSPS-Ⅳ:Fucose:Xyl:Man:Glu:Gal=1.59:0.7:0.38: 1.22:1.93, BSPS-V:Rha:Fucose:Xyl:Man:Glu:Gal=1.16:1.93:0.28:0.21:1.17:1.5.2. Study of blood coagulation system in SD rats of BSPSSD-rats (42), control group, four tested doses of BSPS (100,200,400,800 mg·kg-·d-1) and YN Bai group (100mg·kg-1·d-1).Testing BT and CT,platelet aggregation rate APTT, TT, PT and FIB, the expression of TXB2 and 6-keto-PGF1α. There are statistically significant differences in 400 and 800 mg·kg-1·d-1 as compared with control group, Decreasing BT and CT, increasing the level of rats FIB and TXB2. Decreasing the level of 6-keto-PGF1α.This research reflects that the hemostatic effect of BSPS is reflected by the two ways which includes intrinsic and extrinsic pathway of blood coagulation.3. Protective effect of BSPS against aceti acid-induced gastric ulcerIn this part, rats (49), control group (normal saline), model group, four tested doses of BSPS (100,200,400,800 mg·kg-1·d-1) and Omeprazole group (150mg·kg-1·d-1). The level of PAR1 and EGF mRNA are higher in low group (P<0.01) than control group, EGFR mRNA all have been improved (P<0.01). The level of PAR2mRNA have been improved (P<0.05) in low and middle groups, (P<0.01) in high group. Our results show that BSPS possesses the inhibitory and protective effect on experimental gastric ulcer by increasing the expression of EGF, EGFR and PAR1, PAR2 mRNA in gastric mucosa in rats. In the part, it reveals one of the possible molecular mechanisms of anti-gastric ulcer action, which may be in the way of increasing the expression level of EGF, EGFR, PAR1,PAR2 mRNA in gastric mucosa.