Expression Of SOCS3 In Placenta Of Preeclampsia And Its Effect On Trophoblast Proliferation And Migration

Objective:To investigate the expression of suppressor of cytokine signaling3(SOCS3) gene in the placenta, its role in the pathogenesis of pre eclampsia and its effect on proliferation and migration of HTR-8/SVneo cells.Methods:Fifteen women with severe preeclampsia hospitalized in the First Affiliated Hospital of Nanjing Medical University from October2011to October2012and15women with normal pregnancy during the same time period were investigated.The expression of SOCS3mRNA and protein in placenta was determined by real-time quantitative reverse transcription-polymerase chain reaction and Western blot. Cultured HTR-8/SVneo cells were transfected with SOCS3specific small interfering RNA (siRNA) or negative siRNA as the controls.The expression of SOCS3mRNA and protein in these cells was detected by reverse transcription-polymerase chain reaction and Western blot. Cell proliferation was detected by methyl thiazolyl tetrazolium (MTT), cell cycle by flow cytometry and migration by the Transwell test. Two independent t tests were used for statistical analysis.Results:The SOCS3mRNA and protein levels in the severe preeclampsia group were lower than those in the normal group (0.25±0.03vs0.71±0.08and0.21±0.05vs0.75±0.12, t=15.94and14.29, respectively, both P<0.05). SOCS3mRNA and protein levels in the transfection group at24hours were lower than those in the negative control group (0.39±0.02vs1.00±0.04and0.0037±0.0014vs1.5149±0.0357, t=27.58and73.35, respectively, both P<0.05). The integral absorbance values of cell proliferation in the transfection group at48,72and96hours after transfection were0.23±0.01,0.32±0.02and0.37±0.02, respectively, which were lower than those in the negative control group (0.39±0.02,0.55±0.04and0.86±0.04, t=2.60,6.64and42.44, respectively, all P<0.05). The cell clonal formation was lower in the transfection group compared with the negative group (116±15vs312±24, t=9.96, P<0.05).The ratios of G1/G0and S phase cells in the transfection group were (55.75±2.21)%and (31.59±0.83)%, respectively, and were significantly different from those in the negative control group [(47.88±1.87%) and (37.38±1.34%), t=45.43and20.06, respectively, P<0.05]. After48hours, cell migration in the transfection group was lower than that in the negative control group (93±11vs167±17, t=21.36, P<0.05).Conclusion:SOCS3expression is probably involved in the pathogenesis of preeclampsia by being down-regulated and therefore impeding proliferation and migration of the trophoblast.