| Background Memory is the reflection of experienced things through human brain, which included four basic processes:memorizing, keeping, recognition and recall. Memory loss is generally above the four processes of decline, also known as amnesia. The elderly include memory loss, forgetfulness and functional organic amnesia categories. The elderly due to age memory loss is a common phenomenon occurred, are functional amnesia. With age, age-related neurological diseases occur, especially Alzheimer’s disease as the representative of memory loss diseases, are organic amnesia. In recent years, the number of China’s aging population increased year by year, according to internationally accepted criteria, when the proportion of the population of a city or country reached60years of age or over10%of the total population, entered the aging society, in1999China began to enter the aging society, in2014the country’s aging population of200million.[1]The incidence of learning and memory loss in the elderly-related diseases increased year by year. Forgetfulness is more common disease of the elderly, especially forgetfulness caused by insufficient blood supply to the brain is the most common human brain learning and memory loss as the main manifestation of amnesia. Many of the elderly and their families are experiencing memory loss and suffering caused by cerebral ischemia brings, it also brings huge economic burden on society as a whole.Objective In the medical literature on the basis of research on forgetfulness, Exploration of medicine from the heart, liver and kidney of governance in the knowledge of forgetfulness, with the liver, kidney, qi and blood circulation method to treat forgetfulness, to explore the use of commonly used Chinese medicine treatment forgetful law. On the basis of wisdom soup recipe, combined with clinical experience in the research foundation mentor teacher removed the door to enter into a prescription medicine Tianqi, Tianma, treating diabetes drug group by glutamate damage fetal hippocampal neurons cells, and mouse water maze behavioral observation findings, discuss pseudo-ginseng, Tianma, treating diabetes drug group protection group intervention mechanism for memory loss, which research ideas to restore brain function in elderly patients with health care has a positive meaning.Methods1. Forgetfulness about TCM literature compilation and statistical study on the liver, kidney, the role of qi and blood circulation method in the prevention of forgetfulness.2. Through the establishment of glutamate injury model of hippocampal neurons, research Tianqi, Tianma, the protective effect of treating diabetes drug group Glu damage fetal hippocampal neurons.3. Tianqi, the preventive effect of Tianma, learning treating diabetes drug group for damage caused by scopolamine and memory dysfunction in mice were studied. The mice were grouped experiment, mice were divided into six levels in the normal group received different doses of medicine operator intervention, the control group of mice fed with distilled water, medicine group (low group, the group and the high group), western medicine group each group of mice fed with corresponding drugs, using Morris water maze behavioral test, by detecting hippocampal choline acetyltransferase, monoamine oxidase activity content, explore Tianqi, Tianma, treating diabetes drug group memory in mice protective effects of hypothyroidism.Results(1) One experimental study, MTT method to measure the pseudo-ginsemg, Tianma, the measurement results for treating diabetes drug group Glu damaged neurons OD values and survival, compared with the normal group, the cell viability decreased significantly after Glu injury (P<0.05); pseudo-ginseng, Tianma, treating diabetes drug group protection group dose group compared with Glu damage cells was significantly increased (P<0.05). Tianqi averages, Tianma, the protective effect in treating diabetes drug dose group of Glu damaged neurons and the protective effect of nimodipine group closer than the normal group and the model group.(2) The experimental glutamate in hippocampal neurons injury assay results immune markers of GABA in neurons, the statistical analysis showed that the model group, the average total optical density is lower than the normal group (P<0.05), The average total optical density values were higher than the drug group protection Glu injury group (P<0.05), the normal group, pseudo-ginseng, Tianma, treating diabetes drug group, the mean optical density value close to the nimodipine group, indicating that the protection group GABA levels in the normal group approaching, and higher than Glu injury model group.In marked CAMKII neuronal cells, the statistical analysis showed that the average total optical density model group than the control group (P<0.05), the protection group, the average total optical density lower than Glu injury group (P<0.05) normal group, pseudo-ginseng, Tianma, treating diabetes drug group, nimodipine group close to the total optical density, indicating the amount of the protection group CAMKII receptors close to the normal group, and Glu less damage than the untreated group.(3) A high dose of pseudo-ginseng, Tianma, treating diabetes drug group of normal mice can improve water maze learning outcomes (ie, the escape latency (P<0.05), low-dose group was not significant (P>0.05); and Tianqi, Tianma, treating diabetes drug group and high dose groups of mice hippocampal choline acetyltransferase activity was significantly increased (P <0.01), acetylcholine and Western blot enzyme activity was reduced hippocampal choline acetyltransferase protein expression increased (P<0.05). compared with the control group, scopolamine mice water maze learning and memory performance decreased, hippocampus tissue choline acetyltransferase activity decreased and serum monoamine oxidase activity increased, suggesting that memory impairment model founded Tianqi, Tianma, treating diabetes drug group, high dose group water maze learning and memory performance (ie, after the number of platforms and platform quadrant dwell time) are better than the model group, especially in the high-dose group most significantly; Tianqi, Tianma, treating diabetes drug group and high dose groups of mice hippocampal choline acetyltransferase activity was significantly higher (P<0.05) in:significantly reduced in mice in a water maze escape latency (P<0.05), prolong the time spent in the platform quadrant (P<0.05), and increased hippocampal tissue in mice with choline acetyltransferase activity (P<0.05).ConclusionsOn the basis of excavation of ancient literature, for liver, kidney, qi and blood circulation method to improve memory discussed research to explore effective ways to intervene memory loss, and the advent of the bed nearly10years of traditional Chinese medicine commonly used to improve memory prescriptions were consolidated and statistical analysis. The results found for liver, kidney, qi and blood circulation method commonly used to improve memory in the clinical treatment methods, including high Tianma, pseudo-ginseng, treating diabetes frequency of use, after the election three herbs in the study on the basis of the division gate Tianqi composition, Tianma, treating diabetes drug group to explore the liver,kidney, qi and blood people learn ways to improve the mechanism of memory. The study found that people are affected by a wide range of learning and memory, multi-system impact. Tianqi, Tianma, treating diabetes drug group can achieve modulating neurotransmitter role to protect memory in mice. The results show Tianqi, Tianma, treating diabetes drug group can effectively intervene mice memory ability, through the analysis of the research results of pseudo-ginseng, Tianma, treating diabetes drug group intervention mechanism for memory loss may be through the central bile alkali nervous system and antagonize Ca2+to achieve. Tianqi high-dose group, Tianma, treating diabetes drug group can improve East buttercup if alkali induced memory impairment in mice learning and memory abilities, its mechanism and pseudo-ginseng, Tianma, treating diabetes drug group for improving the hippocampus choline acetyltransferase transferase activity, thus contributing to the synthesis of the brain, reducing the decomposition of acetylcholine related. Tianqi, Tianma, treating diabetes drug group can be adjusted by the neurotransmitter GABA, CAMKII receptor activity plays intervene to protect against glutamate damage hippocampal neurons.
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