Study On The Mechanism Of Double Heart Disease And The Effect Of Yiqi Huoxue Traditional Chinese Medicine Based On Myocardial Ischemia And Emotional Stress Model

Background Along the rapid development of science and technology, the growth of population and the social life’s rhythm speeding up, the traditional interpersonal interaction mode is gradually changing, the pressure comes from work、study、life is added at a rapid pace.More and more sense of urgency、 loneliness troubles human body,leading to heart problems in constriction has gradually into the sight of all kinds of disease research focus on the medical community. In cardiovascular disease,Double heart medicine Cardiology:Spirit, as an important branch discipline of psychosomatic medicine, is causing more and more attention in the process of clinical doctors’diagnosis and treatment disease.Depression, cardiovascular disease are both classed as the increase of the global burden of disease at the same time,both in physical and mental disease in clinical,which caused the researchers a lot of thinking in recent years.After a heart attack of depression and its influence on prognosis of patients received attention The prevention and treatment at the same time spirit-mental illness and cardiovascular disease is double heart medicine.Objective This experiment is under the guidence of double heart medicine theory and TCM holism, disease of heart head blood-vessel qi-blood theory as the instruction, in heart failure rat model, using immunohistochemical microscopic image analysis, protein groups, such as molecular biology technique, by observing the heart failure before, during and after the building of model before and after stimulation, different drugs at different time points on electrocardiogram (ecg) in rats, the change in physical morphology, echocardiography and substance P in the blood of different organs or precursor substance P, endothelin, the content of NO, preserving evaluation analysis of correlation and substance P in cardio-cerebral diseases related to the function and molecular pathway and the effect of yiqi huoxue, explains the importance of the double heart medicine in cardiovascular disease, and provide experimental basis for clinical application of further "watch emotions in heart failure rat model induced by different parts of the related substances in the blood vessel endothelium"Content and the influence of protein expression specificity and the change of the rat behavior, explore the mood in the rat heart cerebrovascular disease prevention and control of the process as well as the role of traditional Chinese medicine (TCM) link, for early intervention to improve myocardial remodeling avoid provides the basis for further developmentMethods1 Experiment 1 Using the method:Celiac-injection of isopropyl adrenaline (5 mg/kg/D)on rats for three days consecutively to manufacture congestive heart failure model.2 Experiment 2 The mood disturbance method, using the black bottle (pressure alone bound), hungry, thirsty empty bottle desire (no) and other methods to stress on the model stimulation in rats, related parameters of different groups of different time points on a comparison of the model of chronic mild stress (CUMS) unpredictability.3 Experiment 3 Irrigate yiqi-huoxue medicine and western medicine to rats. Constrast related indicators in different groups and in different time points.4 Experiment 4 and Experiment 5 Basing on the Experiment 3 to irrigate Yiqi-huoxue medicine and Western medicine to rats. Constrast the amount of ALDH2 SP and Adponectin in heart and brain in different groups.Results1 Experiment 1 Mainly through intraperitoneal injection of the successful completion of myocardial ischemia,myocardial hypertrophy model of rats before and after modeling, compared with sham operation group, model group, Chinese medicine group, western medicine group and blank group rats in heart rate, ECG ultrasound heartbeat changes on the graph:the blank group, the rats in sham operation group had no obvious change, abdominal cavity injection molding part of the rats heart rate changes from fast to slow the situation, after a period of use:model group, Chinese medicine group, western medicine group rats were part of ECG changes, relatively small change in drug group, drug that is to play the role of. Ultrasonic inspection:the model was made thirty-second days after taking drugs, experimental animal of each group were observed ultrasound by examination, echocardiography (IVSTd, LVDd, LVPWTd, IVSTs,LVDs, LVPWTs, FS and EF), the results are sorted, record. Through the analysis found that the model group, drug group LVPWTd, LVPWTs, LVDd increased, LVDs decreased. In the model group compared with the control group and the sham operation group, EF, FS change was significant (P<0.01), intraperitoneal injection of drugs that modeling plays a role. The model group was higher than that of growth and reduce the degree of tendency of drug group. After taking the medicine, traditional Chinese medicine group compared with western medicine group EF, FS values showed an increasing trend, the traditional Chinese medicine has played a good role model, mortality in the controllable range.2 Experiment 2 The establishment of chronic non predictab] e stress on cardiovascular disease animal model. In the early stage of myocardial ischemia, heart failure model, imitation of model human life stress and emotinal stimulation, increased emotional stress. In chronic non basic foresees the establishment and perfusion of Supplementing Qi and activating blood circulation and the positive control drugs, cardiovascular disease and stress on the model of double heart disease, ultrasound, ECG test, open field and sugar water experiment, as well as on heart rate, body weight, discharge times and other related indicators for observation, we found fourteenth days rats have reduced activity, like the dark, fear, secretion grow in quantity, a few days after the loss of appetite etc.. Thirty-second days of cardiac ul trasound in rats, such as experiment, model group compared with the control group, there were significant changes in heart function, compared with the pure P<0.01; cardiovascular disease group, the difference was relatively low, but the existence of a trend. In the open field test and sucrose preference test, experimental results show that, the mood of rats invertical climbing, lateral movement are decreased, curiosity is reduced, the sugarwater partial degree is lower (less than before the start of the experiment, twenty percent) in the observation group showed that emotional stimuli shy, quiet like dark, sucrose preference degree obviously, restless in a strange environment, secretion grow in quantity, to struggle, to reduce the degree of stimulusand other acts, the emotional stimulation to achieve a certain effect. Emtional stimulation effect. Emotional stimulation in rats in addition to the psychology and behavior of rats to produce a certain influence, also has an impact on cardiac function, construct the model of double heart disease successfully, and there is a certain value and significance.3 Experiment 3 With ELISA method to detect the contents of rat serum NO, ET 1, SP. Comparing each index, statistical analysis found that NO both in model group and myocardial ischemia group is obviously higher than that of blank group,while ET-1 is lower than the blank group(P<0.01or<0.05). In Chinese traditional medicine and western medicine group, NO slightly higher than the blank group, slightly lower than the model group, and ET-1,NO higher than that of myocardial ischemia group in the two group,while ET-1 is lower(P<0.01or<0.05).4 Experiment 4 RT-PCR technique is used to inspect rats serum and myocardial cell mitochondria, the hippocampus, SP-mRNA lever in every group. Results showed that the substance P in model group was obviously rise(P<0.01 or< 0.05) in both heart and brain. And there have differences between Chinese traditional medicine and western medicine group in the increasing of substance P content.5 Experiment 5 With RT-PCR technique to detect Yiqi-huoxue medicine for heart failure rat myocardial cell mitochondria and hippocampus ALDH2, lipase mRNA expression Results:the index of complex model group was obviously lower than ISO group and the blank group, medicine groups were obviously higher than that of model group, with statistical significance.Conclusion Yiqi-huoxue prescription may improve myocardial ischemia rats’ cardiac muscle power, myocardial metabolism, to increase their ejection fraction and myocardial blood supply by affecting the content of hippocampus and ALDH2 in myocardial and hippocampus. The prescription may also affect neuromodulation to change the gene lever of SP, NO, ET-1. Further to protect endothelial function, repair the damaged endothelial cells’function, reverse ischemia hypoxia caused by the change of metabolism of cells form function, increase the local myocardial tissue perfusion amount, improve the blood supply of myocardial cells, improve the cardiac function of microcirculation, adjust the blood perfusion of microcirculation. The medcine may affect the content of lipase in myocardial to protect heart, inhibit inflammation, restrain apoptosis, and so on. In addition, Yiqi-huoxue medicine may affect the brain and humoral regulation related factors to change rats’mood, in order to heal the diseaseInnovative This article discussed the correlation between emotion and cardiovascular disease in a microscopic view. Under the guidance of Qi-blood theory and Psycho-cardiology theory,to research the common mechanism of Yiqi-huoxue medicine in healing cardiovascular disease and depressive disorder. The study has clinical research innovation point. Using immunohistochemical methods Rt-pcr to research the relationship between emotion and cardiovascular disease. Discussing the mechanism of Yiqi-huoxue method in treating the myocardial ischemia disease under the guidance of psycho-cardiology theory.The experiment has novelty both in lab-study and clinical application.