Preparation Of Nano - Yam Polysaccharide And Study On Hypoglycemic And Hypolipidemic Activity

Objective Extraction technology of yam polysaccharides was preliminarystudied.Nano yam crude polysaccharides were prepared by ball milling method. Toevaluate the quality of preparation. Through establishment of in vivo and in vitro model,the hypoglycemic,lipid-lowering effect and mechanism of nanometer yam polysaccharidewas studied, it provides the basis for the natural medicine development of diabetes andhyperlipidemia.Methods The hot water extraction and flash extraction process of crudepolysaccharides were extracted from yam, the yam crude polysaccharides were isolated bywater extraction and alcohol precipitation method,determination of polysaccharide contentby Phenol-sulfuric acid method, and research methodology.Nano yam polysaccharideswere prepared by ball milling method,particle size of nano yam polysaccharide wasdetermined with laser particle size distribution instrument. The diabetic rats model wereestablished by intraperitoneal injection of alloxan, continuous gavage for12days, fastingblood glucose, glucose,C-peptide, glycogen levels of rats were measured, body weight,drinking, diet changes of rats were recorded, In order to study effects of nano yampolysaccharide on blood glucose metabolism in alloxan induced diabetic rats.Establishment of the model of hyperlipidemia by feeding the rats with high fat diet for4weeks, Continuous intragastric administration for30days, determination of content of ratserum TC, TG, HDL-C, LDL-C, the influence of nano yam polysaccharide on blood lipidmetabolism in hyperlipidemia rats. HepG2insulin resistance cells model were establishedfor the high concentration of insulin, the consumption of glucose in the culture mediumwas measured by glucose oxidase method, determination of effect of yam polysaccharideon cells proliferation by MTT method, investigate effect of yam polysaccharides on absorbglucose and insulin resistance in HepG2cells.Results Hot water extraction and flash extracting technology of yam polysacchariderate respectively were0.78%,0.53%, the contents of polysaccharide were33.63%,27.38%. Yam polysaccharide samples were stable within2hours, Phenol-sulfuric acidmethod was good reproducibility. Nano yam polysaccharide was prepared on the surfaceof a round, no adhesion, the average particle size was520nm, and good stability. Theresults showed that intraperitoneal injection of200mg/kg alloxan can be successfully induced diabetic rats model. The rats were fed with high fat diet for30days,Hyperlipidemia rats model were successfully induced. The model rats were given nanoyam polysaccharide (50mg/mL,100mg/mL)for12days and30days, yam polysaccharideeach dose groups can significantly lower blood glucose levels in alloxan diabetic rats,improved glucose tolerance, improved glycogen and C-peptide levels, improvedpolydipsia, polyphagia, polyuria and weight loss symptoms in diabetic rats, and cansignificantly reduced the fat rat serum TG, TC, LDL-C levels, HepG2insulin resistancecells model was successfully established in high concentration of insulin. Compared withthe control group, Yam polysaccharide in each dose groups increased glucose consumptionin normal HepG2cells and insulin resistance HepG2cells (P<0.01).Conclusion Preparation of nano yam polysaccharide was safe and reliable, it hasspecial biological effects. Nano yam polysaccharide has obvious regulating glucosemetabolism, improve the symptoms of diabetes, therapeutic effect of regulating bloodlipid, it provides a theoretical basis for the clinical application of nano yam polysaccharideon diabetes and hyperlipidemia.