Study On The Variant New Strain Of Maize Inbred Line Offsprings Received Exogenous DNA

With the methods of seed-immersion and pollen-tube-pathway, the exogenous DNA extracted from different plants is introduced into different maize inbred lines. The progenies appear various mutant types and the mutant characters are very abundant, mainly including the quality of field growth such as the height of plant, the height of fruit the number of leaf the color and morphology of leaf the length and width of leaf the area of leaf the weight of 100 grain and the period of growth etc. These varied characters produce the new ones , which tend to be stabilized in D₃ generation . And analyses peroxidase isozymorgame chlorophyll content index of nutrition. Through three years planting , synthesize various measurement index and agronomic character , Some stable heredity and fine mutant material have been selected , the results are as follows:1 .The seed-immersion methodThe total DNA of donor from soybean is introduced into waxy corn 12-9-10 inbred lines with the density of 300μg/ml. The mutant rate of D₀ generation is 7.74%.Analysis of peroxidase zymogram, the results show peroxidase zymogram of mutant plant is littler than ck for 2 zymogram bands in RF=12 and RF=13 , and the others are the same as receptor. Through observing character of 4 generation from D₀ to D₃ , the height of plant the height of fruit and area of leaf are all variety. The height of plant of D3-10(2) is lower than ck which is 117cm for 3.67cm ; Area of leaf of D₃-10(1) is higher than ck which is 203.99 cm² for 229.04cm². But because andr is...