| Potato clones with desirable chipping traits were breeded and selected at diploid level, as well as to construct a molecular linkage maps based on AFLP technology by using a diploid segregating population, so that the chipping traits could be improved by the molecular marker-assisted selection. Furthermore, it would be ready for mapping and isolation of the genes or main effect QTLs about chipping traits. The results were as follows:1. 9 clones of diploid potato with light chip colour were screened out after the evaluation during 2003～2005, and are 02018-32,02018-44,02018-93,02018-99,02018-105,02018-179,02018-221,02018-242 and 02018-248.2. 5 diploid clones of 02018-93,02018-94,02018-105,02018-176 and 02018-248 with good cold chipping qualities were obtained after the two-year evalution under 4℃/6℃cold-storage and reconditioning at 18℃. The experiment results also showed that tubers of clones from 02018 population stored at 6℃for 80 days were much easier to be reconditioned than those-stored at 4℃for 90 days.3. A molecular linkage map of potato (Solanum tuberosum L.) was first developed in China based on amplified fragment length polymorphism (AFLP) markers by using a F1 segregating population from the cross between the diploid potato parents of 08675-21 and 09901-01. The genetic map included 24 main linkage groups, and the female-maps covered 512cM with an average distance of 6.83cM between adjacent markers while the male-maps cover 578cM with an average distance of 6.08cM between adjacent markers. |
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