Construction Of A High-density SNP Genetic Linkage Map Of Colored Potato And QTL Mapping For Important Traits Such As Anthocyanin Content

Potato（Solarium tuhero.sum L.）plays an important role in ensuring national food security and meeting the needs of human health for food structure adjustment.Colored potatoes not only have the nutritional value of ordinary potatoes,but also have a high content of anthocyanins,which can be used as one of the important raw materials for extracting natural pigments.Because anthocyanin has strong free radical scavenging ability and anti-oxidation,it has good utilization value and development prospect in food processing,pharmaceutical and cosmetic additives.Due to the late start of research on colored potatoes,there is currently a shortage of colored potato varieties on the market.For fostering high anthocyanin content,high yield and strong resistance characteristics of excellent color potato varieties,this experiment with four times the body colour potato"Red-P1"as the female parent,"black beauty"as the male parent,artificial emasculation bagged hybridization experiment was carried out,get the color separation plant potato hybrid F₁generation,then through intraspecific selfing generation 130 F₂segregation population.In this study,130 isolated plants and their parents from the F₂generation of tetraploid color potato were used to develop SNP markers using SALF-seq high-throughput sequencing technology.The SNP high-density molecular linkage Map of color potato was constructed by Jion Map 4.0 mapping software.On this basis,Map QTL6.0 software was used to study QTL mapping for tuber anthocyanin content,starch content,dry matter content and tuber yield per plant of color potato.This experiment can lay a foundation for further studies on QTL fine mapping,gene function analysis and molecular marker breeding for yield traits and anthocyanin content of color potato.Sing SLAF-seq high-throughput sequencing technology,developed 4167 high-quality SNP molecular markers for color potato genetic linkage map mapping.The main results were as follows:1.Using SLAF-seq high-throughput sequencing technology,4,167 SNP molecular markers with high quality were developed for color potato genetic linkage mapping.2.Construct a high density tetraploid color genetic linkage map of potato,containing12 linkage groups and 4,167 SNP markers,covering potato genome length of 2143.36 c M with an average map distance of 0.51 c M.3.According to the Chi-square test,353 of the 4,167 SNP markers were partial markers,with a partial separation ratio of 8.47%and a partial separation ratio of<30%,which met the requirements of partial separation markers in the construction of plant genetic linkage map.4.Phenotypic values of anthocyanin content,starch content,dry matter content and tuber yield per plant of tetraploid color potato were measured and their normal distribution test and correlation analysis were carried out.The results showed that the phenotypic data of the four traits were normally distributed in different environments,which was consistent with the genetic characteristics of quantitative traits and could be used for subsequent QTL mapping analysis.There was significant difference and correlation among the characters to some extent.5.Based on the constructed genetic linkage map,four important traits of color potato including anthocyanin content,starch content,dry matter content and tuber yield per plant were mapped by QTLs.A total of 11 stable QTLs were detected,which were located on LG05,LG06,LG08 and LG11 respectively.Among them,four major QTLs were q Ac8,q Dm6,q Ty5-1 and q Ty5-2,and the genetic contribution rate varied from 10.89%to24.06%.