Physiological And Biochemical Research In The Germinating Process Of A.albus Seeds

Elephant-foot yan, Konjac is a perennial herbaceous plant of Amorphophallus Blume, Araceae, Monocotyledoeae, and is mainly distributed in India, Chinasouth Africa peninsula, and southern China of Asia and Southeast Asia. At present, there are 163 species discovered all over the world. In China, there are 21 species, of which 9 species are peculiar to our country, mainly distributed in mountainous areas and plateaus of urban districts in each province to the south of Qinling Mountains. A, konjac K.Koch and A. albus Liu et Chen are two species that are cultivated in China on a large scale for economic purposes, and both contain KGM as their economic ingredients. A. albus is a new species that is discovered and named by Liu Peiying and Chen Jinfeng in 1984, peculiar to our country, and it is one of main cultivated species. The natural distribution region of A. albus is Jinshajiang Stream Valley Zone, mainly concentrated in the south of Sichun Province and the north of Yunan Province.The propagative methods of A. albus are classed into two kinds, self-propagation and artificial propagation. Self-propagation mainly includes asexual propagation of asexual organs, rhizome and tuber, and sexual propagation of seedling seed. While artificial propagation includes tissue culture, slab propagation, off-bud propagation and other asexual propagation methods. Because Konjac adopts asexual propagation for a long time, the phenomenon of miscellaneity and degeneration of its later generations is very serious. This affects the outputs and quality of Konjac directly. Making use of seedling seed propagation not only can play heterosis, increase the ability to resist disease, and improve propagation coefficient, but also can avoid seed tuber degeneration. So it is an effect way of settling virus-free propagation of Konjac seed tuber and avoiding degeneration. But the dormancy stage of Konjac seedling seed under field conditions is longer, and the rate of emergence is extremely low, which is often below 30%, so it is hard to use directly in production. Therefore it is essential to know the physiological-biochemical change of every growing period of Konjac seed germination.1. Break dormancy of seed and facilitate germinationThe dormancy of A. albus seed is physiological dormancy, and its dormancy stage is over 150 days. Orthogonalty design L9 (34) is used to study the effects of different concentration (factor B) of four kinds of hormone (GA₃, IBA, BA, KT, thiourea) for different treatment time (factor C) on germination rates of three materials (factor D, rhizome, seed stored at 5°C and 20 °C respectively) at different germination temperature (factor A, 20°C, 25°C, 30°C). Germination rates of nine treatments are recorded after 40 days in order to find the optimal combination. The results as follows: the...