Preliminary Study On Burl Of Chinese White Poplar Forming's Anatomic Feature And The Test For Agrobacterium Tumfacience Distribution In Chinese White Poplar

Burl Wood has been extensively applied to decorate the costful furniture, and it also has been made various things of arts and crafts.However it is restricted that trees develop Burl Wood ,result from the forming particularity of the wood texture .In order to dispose the shortage of Burl Wood ,the resceach taken the male Chinese White Popular( populus tomentosa )of tissue culture as the material ,and taken advantage of infecting characterstic of the Agrobacterium tumfacience, contransformed T-DNA of the wild Agrobacterium tumfacience to the male Chinese White Popular.The regeneration system of the trans-inducing crown gall gene were set up.The stem and crown's anatomic was observed. Last we tested Agrobacterium tumfacience Distribution in plant ,after Agrobacterium tumfacience inoculated on the male Chinese White Popular. The main results are as follow:1. Through the observation and comparison the crown gall's anatomic structure at fifth day and thirtieth day , we discovered that the cambial cell and primary xylem cells were very sensitive when the stem was inoculation by wild Agrobacterium tumfacience. Cambial cell divided magnificent thinwalled container outwardly;Xylem tissue divided a large number of thinwalled cells inwardly too .It is shown that vascular bundle was the initial site of the cell division . Cell division made a difference on the structure of the stem. The transverse section of the hoster stem taken on abnormal ring-shape. Several obvious pith ray can be seen ,whereas there were only a few or illdefined pith ray .The host's vascular system developed rapidly at the inoculation site, then formed larger gall outwardly, the basipetal direction , increased vascular bundle contained increasing vessel size. In the centripetal direction the increased vascular bundle contain reduction vessel size.There were a bundle of xylem tissue and phloem tissue in the crown gall, in which there contained tree-like branch bundles in fast-growing xylem region ,and spiral vascular tissue in phloem tissue .2. The male Chinese White Popular was infected by strains I ,1-1-2,III,the result shown that 1-1-2 possessed the strongest virus. Inducing rate was 90.75%. Crown gall could grow normally in the culture medium without any auxin. Culture medium MS adding TDZ0.1 mg/L +IBA1.0mg/L was the best one, the rate of inducing crown gall was the highest.Transform plant was confirmed by the PCR and agropine analysis. The result shownthat T-DNA of Ti plasmid had been combined into the regeneration plantlet.3.Inoculation the stem of the male Chinese White Popular of tissue culture by strainl-1-2, after one month ^igrobacterium tumfacience were divided respectively from the roots, stems, leaves and crown gall. It was confirmed by PCR and agropine analysis that the divided strain was the inoculatedl-1-2 strain, which shown that aimed strain has been pervated plant's root and leaves from cut site .But the result was not consistent with the field test, the strainl-1-2 only was detected from crown gall ,none was detectct from other organizations. Therefore the result must be test further later.