Study On High Protein Feed Made By Multi-strains Two-step Co-fermentation

In this experiment that researches the effect of several bacterium to the corn straw,for making high protain straw feedstuff.This study not only settles the pollution of circumstance by burning straw,but also has large economy benefit.In the paper that introduce the large benefit for society and condition of making straw feed, also recite the technic and ways to deal with straw.Discussing the question and hotspot of disposing.This experiment of the first part design adopted four kinds of substrates, transfering P-1, L-1 respectively to four kinds of substrates: Ⅰ the kernel substrates, Ⅱ the straw powder substrates, Ⅲ the sawdust substrates, Ⅳ the corn powder liquid substrates, observe its growth condition, the result is the two germes of growing in the kernel substrates and the corn powder liquids substrates to be prosperous, growth speed quickly. And transfer above-mentioned substrates of seed inoculates the straw powder substrates, examining the development speed of the hypha, the thick protein and the fat contents, as well as the growth speed and ultimately production, its result the production that regards kernel as the component of substrates and the production that regards the com powder as the component of substrates in the straw powder substrates , its total development speed is quickly, incubation is shorter, the thick protein content is higher, for example the straw substrates contained with 70% water, its final thick protein content amount to 23.79%, the straw substrates contained 65% water inoculate the corn powder seed, the final thick protein content amount to 21.87%.After consider,selecting corn powder substrates as seed substrates.Experiment of the second part researches the cellulose enzyme vitality of bacillus FS by thelaboratory screened , and also observing its momophological characters, measuring thephysiology biochemical characteristic and the conten of the DNA G+C mol%, according《 the authenticates manual of the familiar germs system 》 of Dong Xiuzhu, the first stepidentified that germ to belong to bacillus.Experiment of the third part use the first part's production as carrier, selecting Geotrich candidum and Candida tropicalis of higher production of protein also selecting producing the cellulose enzyme Bacillus subtilis FS and Lactobacillus bulgaricus by ourselves separated, design orthogonal test and a series of single factor experiment, finally assurancing the inoculation quantity of four strains germ, is Geotrich candidum 5%, Candida tropicalis 5%,Bacillus subtilis FS 5% of FS, Lactobacillus bulgaricus 3%, the most suitable development temperature is 30 "C , the development time is 15 days. After the second incubation, the production of thick protein content amount to 45.63%. thick fat content amount to 0.92%, thick fibre reduce to 17.23%.