Effect Of Specific High Immunized Vitelline Fluid To Chickens E.tenella

In order to study the characteristics of humoral immunity on E.tenella . Through the preparation of specific vitelline antibody of E.tenella, determine the metabolic pattern of vitelline antibody on caecal. With the internal and external restraining and killing experiment of E.tenella, examine effect of specific high immunized vitelline antibody on E.tenella. Indication of results: (1) Using Dot-ELISA after oral administration of vitelline antibody determination of antibody titer at caecal IgY. It is decreased from original 10.75 to 7.75. (2) Indication of internal experimental result: The Specifitic IgY has no evident effect on the speed of sporation of coccidia, and also has no effect on pathogenicity oocyst of sporation clinical result is obtained from the treatment of 3ml per time and one time per day, the treatment from the same time as that of challge into it. The result obtained are ACI increased to 73.50, weight increased by 39.19%, mortality decreased by 33.4%, OPG raised by 172.2%. Using the treatment of 2.5ml per time and 2 time per day, the results obtained are ACI raised to 92.04, body weight increased by 56.75%, lethality decreased by 33.4%, OPG increased by 66.7%. The difference between 1 time per day and 2 time per day is extremely remarkable (P<0.01). (3) Indication of external experimental result: Vitelline antibody has no effect on the speed of sporation of coccidia, and also has no effect on pathogenicity coccidia, but it has significant dissolving and weakening effect on sparozoite when the effect goes on for 12 hours, ACI increased to 78.37, mortality decreased by 22.2%, body weight increased by 53.25%, OPG raised by 112.7%. The difference of 24h and 12h is not remarkable (P>0.05). (4)The result of histology showed: vitelline antibody has very good restraining and dissolving effect to coccidia when it is at sparozoite and merozoite stage, it can decrease the invasiveness of coccidia on caecal epithelial cell. When the coccidia is being at merozoite and gametogony stage, vitelline antibody has not clinical effect on it, and it cause injury of caecum..