| The compound immunoenhancer of traditional Chinese medicine which studied in this article,can notably increase little animal live rate,lowering the morbidity.strenghen the organism immunity function through clinical application. Research shows that the polysaccharide is the main valid composition of the compound to strengthen immunity function. But the mechanism is not clarity ,this experiment is to notify the immunity function and mechanism of the polysaccharide of Chinese herbs composite and its single Herb of G-Immunoenhancers.Preparation the serum from the total Polysaccharide of Chinese herb recipe and water decoction, each single herb polysaccharide,normal saline enema after oral administration. Determination lymphocyte proliferative and nitric oxide(NO) production of peritoneal macrophages.Reverse transcription-polymerase chain reaction (RT-PCR) and gel documentation analysis system were exploited to detect the IL-2 mRNA expression levels. Results:1 All of the polysaccharides and water decoction can increase lymphocyte proliferate in differ degree,it explains that thesee medicament have effect on immunity function.2 Ten kinds of medicated serum can increase lymphocyte proliferate in different degree, but there are three kinds of polysaccharides of single herbs without such effect, it is possibily caused by some efficiency component can not absorb from the alimentary canal.3 The medicated serum of water decoction and four kinds of polysaccharide, and these medicament itself can notability enhance NO creation,the result means that these polysaccharides can markedness improve the function of M Φ ,strengthen the recovery function of peritoneal of mice.4 All of the polysaccharides and water decoction can increase the expression of IL-2 mRNA,enhance the body's immunity level.This is one of the mechanism of these medicines.5 Synthetize these results, the total polysaccharide of this composite had the best effect on immune function than all the other medicines. The immunopotentiating effect of total polysaccharide may be associated with its potentiation of IL-2 mRNA expressions at the transcriptive level ,enhance lymphopoiesis and increase creation of NO. |
