| The paper included three experiments which studied the requirements of vitamin C for juvenile Chinese sturgeon (Acipenser sinensis), effect of dietary vitamin E supplementation on growth and tissue vitamin E content of juveniles amur river sturgeon(Acipenser schrendkii) and the requirements of vitamin A for juveniles amur river sturgeon(Acipenser schrendkii) respectively. The single factor gradient testing method was adopted in all the experiments. Six levels of vitamin C were added to the feed of Chinese sturgeon. Seven levels of vitamin A were added to the feed of Amur River sturgeon. As for vitamin E, seven levels were added to the feed of Amur River sturgeon. The average rate of weight gain, survival rate, specific growth rate, feed efficiency, composition of muscle, concentrations of vitamins in liver and muscle, activities of lysozyme and super oxide dismutase (SOD) in the serum of juvenile Chinese sturgeon (Acipenser sinensis)and juvenile Amur River sturgeon(Acipenser schrendkii) were used to evaluate the optimal dietary vitamin requirement. The results were listed as follow: 1. Study on the requirements of Chinese sturgeon(Asperser sinensis)juveniles on vitamin CAn experiment to determine the dietary vitamin C requirement of juvenile Chinese sturgeon (Acipenser sinensis) was carried out. Six diets containing 0.5 (control diet),59.8, 119.5, 239.1, 478.1,956.2 mg vitamin C/ kg diet(C0.5,C59.8C119.5,C239.1, C478.1 and C956.2) respectively were used to fed the fish for 8 weeks. The optimal dietary vitamin C requirement was evaluated based on growth performance, tissue vitamin C content and some related enzyme activities. The results indicated that fish fed C0.5, C59.8 diets showed lower average rate of weight gain, feed efficiency and protein efficiency ratio (PER) than that of the C1195 diet (P<0.05). While increasing dietary vitamin C content from 119.5mg/kg to 956.2 mg/kg these results didn't change significantly (P>0.05). Fish fed the C239.1 diet showed the highest muscle and liver vitamin C content. The highest activities of lysozyme and super oxide dismutase (SOD) were found at the C239.1 diet. According to non-linear regression, the best weight gain was obtained at the dietary vitamin C level of 100.2mg/kg~108.8mg/kg. The highest accumulation in liver at the dietary vitamin C level of 273.3mg/kg~309.4mg/kg.2.Effect of dietary vitamin E supplementation on growth and tissue vitamin E content ofjuveniles antur river sturgeoa(Acipenser schrendkii)An experiment to determine the dietary vitamin E requirement of juvenile Amur River sturgeon (Acipenser schrendkii) was carried out. Seven semi-purified diets containing 0.01 (basal), 2.04,5.10, 10.09, 20.14, 50.05,100.60mg vitamin E/lOOgdiet (Ei, E2, E3, E4, E5, E& E7) were used to feed the fish for 56 days. The result indicated that dietary vitamin E content had significant effect on weight gain, survival rate, feed efficiency, specific growth rate, and tissue vitamin E content of the juvenile Amur River sturgeon (P<0.05). The fish fed by E5 got the highest feed efficiency, specific growth rate, fullness index of the carcass than other groups, such values of E5 were 108.35%, 3.96, 0.75 respectively; The fish fed by E4 got the highest protein and the lowest moisture and lipid contents of the muscle, the values were 16.86%, 77.50%, 4.96% respectively. The liver and muscle vitamin E contents were higher in E6 and E7, E6 were 7.083ug/g andll8.347ug/g respectively, E7 were 7.405ug/g and 121.395ug/g respectively. The fish fed by E4 got the lowest hepatosomatic index (3.23). However excess dietary Ve (100.60mg/100g) had negative effects on the growth and Ve retention. E7got relatively low feed efficiency (93.77%), specific growth rate (3.69), fullness index (0.65), and carcass protein content (16.17%). Therefore the optimal deitary Ve content of juvenile Amur River sturgeon was 20mg~50mg/100g diet. 3.The requirements of juveniles amur river sturgeon {Acipenser schrendkii) on vitamin AThe trial was conducted to evaluate the effects of different dietary vitaminA levels(added vitaminA 10, 258, 510, 1050, 2020, 4100 and 8300IU/kg respectively to the basal diet) on growth performance, muscle composition, liver and muscle vitamin A concentrations, activities of lysozyme activities and super oxide dismutase (SOD) in the serum of juvenile Amur River stargeon(Acipenser se/wwK/Jh7)(10.25±0.83g). The results showed that the optimum requirement of vitamin A for the juvenile Amur River sturgeon was 1000IU /kg diet, which could get the highest growing rate, survival rate, weight growth rate, feed efficiency, specific growth rate and fullness index of the Amur River sturgeon. The muscle composition didn't change significantly (P>0.05). But the fish fed on the diet with 1050IU VA/kg diet had the highest muscle protein content and the lowest lipid and moisture content. If the content of vitamin A was lower or higher than 1050IU in diet of 1000g, which could reduce the growing rate, survival rate, weight growth rate, feed efficiency, specific growth rate and fullness index. The activities of lysozyme activities and super oxide dismutase( SOD)in the serum were the highest when the content of vitamin A was 1050IU/kg diet. Either deficit or excess(>1050IU/kg)of vitamin A in the diet would reduce the activities of the...
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