| Siraitia grosvenorii (Swingle) C. Jeffrey is a species of Siraitia in Cucurbitaceae, and it is a dioecism and a famous Chinese medicine plant unique in our province worldwide.Molecular marker has been widely used in classification, cultivars identification, genetic mapping, breeding and location of target gene in plants in recent years. But there were few studies in S. grosvenorii in the world. RAPD(Random Amplified Polymorphism DNA) and AFLP(Amplified Fragment Length Polymorphism) were employed to detect molecular markers linked to sex in S. grosvenorii by BSA (Bulked Segregant Analysis) . The results were as following:1. The genomic DNA of S. grosvenorii with high concentration and purity could be obtained with the improved method of SDS, which was good enough for RAPD amplification. And the genomic DNA can be completely digested.2.18 RAPD markers linked to sex were selected in BSA by screening 90 primers. Only the marker amplified by S1431 was present in all 8 male individuals tested while absent from all 8 female individuals tested. It showed that it was a male marker closely linked to sex. This work has provided a basis for cloning sex-linked gene.3. AFLP technique was employed to study 5". grosvenorii' s sex identification. And an AFLP technique system suitable for S. grosvenorii was established and optimized. AFLP bands were clear and steady in map. It will be the basis for the use of AFLP in S. grosvenorii. |
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