Biological Characters Of Monocytes In Peripheral Blood And Its Relation With The Disease Resistance In Chiken

Monocytes are one kind of important cells in the non-specific and specific immunological responses to protect organism from invasion of bacteria and virus. 8 Silkies and 8 Starbros were selected randomly to study the relationship between the monocyte functions and the disease resistance. Mononuclear cells were obtained by the density-gradient centrifugation of peripheral blood in lymphocyte separation medium and cultured in the common conditions. After staining with AO and Giemsa and labeled CD14 antibodies, the large amounts of adherent cells (about 90% and a week later almost 100%) were proved to be monocytes which adhesiveness, phagocytosis, the activity of lysosomes and cytotoxicity were tested.The adhesiveness, phagocytic index for phagocytosis, the activity of lysosomes and the cytotoxicity in monocytes which were 26.85%±8.24%, 370.33%±l74.57%, 2.47±0.26, 85.05%±6.03% respectively in silkes, were significantly higher than the 18.34%±8.15%, 197.04%±30.95%, 2.20±0.22, 75.68%±5.49% in Starbros respectively(P<0.05). After correlation analysis, the cytotoxicity showed a close correlation with the adhesiveness, phagocytic index and the activity of lysosomes, and the correlation coefficients were 0.54, 0.80, 0.67 respectively(P<0.05). The correlation coefficient between cytotoxicity and the phagocytic index was the highest one. It was considered that the cytotoxicity of monocytes and the phagocytic index could be used to embody the animal immunity.Pullorum Salmonellas which is a kind of Gram negative bacteria can lead to diseases in different age of chickens. Two group Silkies with different phagocytic index were selected after the phagocytosis test, then each group 10 individuals were selected at random from the chickens being negative by giving-out-bacteria and serological test. Two group chickens were injected hypodermically with Pullorum Salmonellas 10~7/ml 0.2ml for the first time and 10~9/ml 0.2ml for the second time. The result displayed the weaker monocyte phagocytosis group had the more obvious symptom. According to the antibody level, the stronger were significantly higher than the weaker, whose agglutinability were 50.21 ±6.67 and 16.85±4.52 respectively (P<0.01), and for the first and the second bacteria injection the stronger group could produce antibodies faster and more. For the body temprature test, the result that was the stronger had the more intense change than the weaker suggested that monocytes in these chickens have the higher ability. During this experiment, the stronger had the lower amount of giving-out bacteria (168.98×10~8±294.74× 10~8) than the weaker (306.52× 10~8±467.97× 10~8) (P<0.05) . The change of the body weiht between the two groups hadn' t shown the statistical difference although the body weight fall of the stronger was lighter. This experiment indicated the phagocytosis of the peripheral blood monocytes could display the animals' disease resistance, and could be a promising cytological index to improve the the disease resistance in animal production.