The Effects Of SPI And DSBM On Protein Retention And Intestine Proteinase Activity Of Juvenile Jian Carp(Cyprinus Carpio Var.Jian)

Two trials were conducted to study the effects of soybean protein isolate(SPI)and dehulled soyabean meal(DSBM) on protein retention and intestinal proteinase activity of juvenile Jian c&rv(cyprinus carpio Var. Jian) with body weight 10-35g. The effects of SPI and DSBM on the protein retention and intestinal proteinase activity of juvenile jian carp was studied in trial 1 and trial 2, respectively. In trial 1, 720 juvenile Jian carps with initial weight 11. 34?.16g were averagely allocated to 5 treatments with 3 replications , feeding diets with fish meal protein substituted by 0, 40, 60> 80 ft 100% SPI protein respectively. In trial 2, 720 juvenile Jian carps with initial weight 10.29+0. lOg were averagely allocated to 5 groups with 3 replications, feeding diets with fish meal protein respectively replaced by 0, 25> 50, 75 ft 100% DSBM protein.In trial 1,intestine weight, intestine length, height of proximal intestine folds, protein efficient ration(PER), productive protein value (ppv), hepatopancreas weight, epatopancreas index(HI), trypsin and chymotrypsin activity in hepatopancreas and intestine, were significantly affected by the dietary SPI level(p<0.05 or p<0.01). Intestine weight, intestine length, height of proximal intestinal folds , PPV, PER, and proteinase activity significantly decreased with the dietary SPI levels increasing(P<0.05), while HI, increased (P<0.05) .There was a significant reduced PPV in fishes given diets with fish meal protein substituted by SPI protein over 60%(p<0. 01). The trypsin and chymotrypsin activity in the hepatopancreas , were negatively correlated with SPI level (rl=-0. 9642, p<0. 01, r2=-0.9391, p<0.05), trypsin inhibitor activation(TIA)(rl=-0. 9675, p<0.01, r2=-0. 9422, p<0. 05), but postively correlated with feed intake (rl=+0. 8764, p<0. 05, r2=+0. 8580, p<0. 1). The intestinal trypsin and chymotrypsin specific activity were negatively correlated with SPI level(p<0. 05 or p<0. 01)and TIA(rl=-0. 9536, P<0. 05, r2=-0. 9471, P<0. 05), but positive correlated with feed intake too (rl=+0. 9354, P<0. 05; r2=+0. 8416, P<0.1). PER and PPV were negativelycorrelated with SPI level (rl=-0. 9761, p<0. 01;r2=-0. 8906, p<0. 05), and positive correlated with pancreas trypsin, chymotrypsin specific activity (PER:rl=+0. 9790, p<0. 01, r2=+0. 9521, p<0. 05. PPV:rl=+0. 9078, p<0. 05, r2=+0. 8130, p<0. 1), and intestinal trypsin and chymotrypsin specific activity (PER:rl=+0. 9766, p<0. 01, r2=+0. 9658, p<0. 01, PPV: rl=+0. 9044, p<0. 05, r2=+0.8130, p<0.1). There was also negative correlation between feed conversion(FCR) and PER, PPV (rl=-0. 9800, P<0. 01, r2=-0. 9170, P<0. 01 ).In trial 2, PER, PPV, HI, hepatopancreas-secreting proteinase, specific activity, and intestinal proteinase specific activity, were significantly affected by the dietary DSBM protein level (P<0. 05 or P<0. 01). Intestine weight, intestine length, height of proximal intestinal folds , PPV , and proteinase activity significant decreased (p<0.01), HI , PER decreased significantly also(p<0. 05) with the dietary DSBM level increasing. There was a reduction in PPV when the fish meal protein was substituted by DSBM up to 50%( P<0. 01). Trypsin and chymotrypsin specific avtivity in hepatopancreas were negatively correlated with DSBM level(rl=-0. 9494 , P<0. 01 ; r2=-0. 9801, P<0. 01), TIA (rl=-0. 8927, P<0. 05 ; r2=-0.8752 , P<0. 05), but positive correltated significantly with feed intake (rl=+0. 9467, P<0. 05 ; r2=+0. 9061,P<0.05). Intestinal trypsin and chymotrypsin specific activity, were negatively correlated with DSMB respectively(rl=-0. 9623, P<0. 01; r2=-0. 9759, P<0. 01), TIA (rl=-0. 9593, P<0. 01; r2=-0.9341, P<0. 05) , but positive correlated with feed intake (rl=+0. 9346, P<0.05; r2=+0. 8247, P<0. 1). PER, PPV were negatively correlated DSMB ( rl=-0. 9761, P<0.01 ; r2=-0. 8906, P<0. 05 ) , but positive correlated with pancreas trypsin and chymotrypsin specific activity (PER: rl=+0. 9605, P<0. 01; r2=+0. 9693, P<0. 01; PPV: rl=+0. 9776, P<0. 01; r2=+0. 9782, P<0. 01), trypsin and chymot...