| The experiment was conducted to study the effect of insitol deficiency on pathological changes, digestive and immune function of juvenile Jian Carp (Cypdnus carpio Var. Jian). 360 juvenile Jian carps(initial weight 10.31±0.04g) were randomly allocated to 2 groups feeding the purified diet containing insitol 450 mg/Kg, feeding the respectively for 80 days. During trail stage, insitol deficiency symptoms in juvenile Jian carps were observed, such as eyes hyperaemia, body surface and caudal fin erosion. The another symptoms, such as entire internal organs dull, atrophy yellow brown hepatopancreas and thinner and more white intestinal tract. The insitol deficiency significantly decreased juvenile Jian carps weight gain, bait intake, the length and weight of intestines, pancrea, hepatopancreas weight/body weight and intestines weight/body weight, intestines weight and intestines weight/body weight(P＜0.01), markedly increased the influence of bait coefficient(P＜0.01). The insitol deficiency significantly decreased the protein content of hepatopancreas and intestines decreased. The activity of intestine trypase, chymotrypsin, lipase, entero-amylase, alkaline phosphatase, Na+, K+-ATPase,γ- GT and creatinkinase were all significantly decreased(P＜0.01). The insitol deficiency markedly decreased the activity of serum lysozyma, the level of serum agglutinin, total iron binding capacity, significantly decreased the content of complementC3,C4 and IgM(P＜0.01), decreased the survival rate 38.33％and 20％before or behind eliminating toxic material. The kidney, spleed weight/body weight, the number of blood akaryocyte, leucocyte were also significantly decreased. the results showed that: the typical insitol deficiency symptoms were eyes hyperaemia, body surface and caudal fin erosion, entire internal organs dull, atrophy yellow brown hepatopancreas and thinner and more white intestinal tract. The insitol deficiency made decreased digestion and absorbing ability through dysplasia of digestive organs and decreasing the enzymes activity that relating to digest and absord. The insitol deficiency made decreased innate immunity through by decreased serum lysozyme activity, the level of lectin, serum total iron-binding capacity, serum complement C3 contents and serum complement C4 contents. The insitol deficiency made decreased adaptive immunity through hindering the growth and development of immune organs, decreasing the number of blood leucocyte and akaryocyte, the content of IgM. The decreased ability of resisting diseases finally made decreased survival rate, before and after challenge.
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