Influence Of Yucca Schidigera Extract On Rumen Fermentation And Diet Nutrient Digestion In Sheep

Twelve male adult sheep with permanent rumen cannula were used in a single factorrandomized block design to study the effect of four levels of YE (0mg/㎏,100mg/㎏,200mg/㎏,300mg/㎏) on rumen fermentation items(pH,VFAconcentration,enzymes activity andthe number of protozos),rumen digestibilities (48h) and apperent digestibilities of OM,DM,ADF,NDF,blood biochemical items. Trial 1. Rumen liquid was sampled at 0h,2h,4h,6h and 8h after feeding. pH,VFAconcentration were mensurated.The results showed that pH were not distinctly influenced forfour groups at the same time points. The pH of all levels were in normal range(pH5.5-7.5).The variety trends of pH have delayed as the concentration of YE supplementincreasing. With the increaing of YE level,the concetration of acetic decreased accordingly,but thepropionic concetration increased.YE at level of 300mg/kg, compared with control ,the aceticconcentration decreased by 15.1% and 19.8% at 4h and 6h after feeding, respectively.Thediffereces of concentratins of YE did not significantly influenced the concentration of totalvolatile fatty acids(TVFA). Trial 2. Enzymes activity and number of protozos of ruminal liquid were measured at 2hafter feeding at every seven days during the twenty-eight days. According to the results of thisfive days, the changing trends of activities of Total dehydrogenase and Cellulase wereincreased as the level of Yucca asponins increasing. Cellulase activity was distinctlyinfluenced at level of 300mg/kg compared with control and 100mg/kg group .The the numberof protozos in every group has no distinct regulation,but there were decreasing trends as theYE level increasing. The number of protozos of 300mg/kg YE was distinctly influencedcompared with control and 100mg/kg group. Trial 3. The effect of the three levels of YE on the rumen degradability of DM,OM,NDFand ADF in 48h were evaluated with nylon bags. The results showed that the trends ofdegradability of nutrient material increased as YE supplement incresing. Rumen IIAbstractdegradability of OM,DM,NDF in 48h for 300mg/kg YE was distinctly influenced comparedwith control and 100mg/kg group (P<0.05) ,but the rumen degradabilities of ADF in 48h infour groups were not distinctly influenced. Trial 4 . In this experiment the effect of three levels of YE on the apparent digestibility ofADF were studied.The results showed that four groups were not distinctly influenced bysupplement of YE(p>0.05). Apparent digestibilities of DM,OM,NDF had decreasing trendsas the increasing of YE.Apparent digestibilities of DM,OM,NDF for 300mg/kg YE weredistinctly influenced compared with control group (P<0.05). Trial 5. The results showed that the blood biochemical items of four groups were notdistinctly influenced by YE supplement(p＞0.05). All blood biochemical items were innormal range.The concentration of urea-N in serum decreased as the the increasing of YE.Theactivities of AST,GGT,ALP,LDH,CRE were not distinctly influenced. Candidate:Liu Chunlong Specialty:Animal nutrition and feed science Supervisor::Prof. Li Jie...