Studies On Male Sterility-associated Special Proteins In Xanthoceras Sobifolia Bunge.

Xanthoceras sorbifolia Bunge. is the specific oil plant in north of China and has great officinal value. The flower of Xanthoceras sorbifolia Bunge. is polygamous. The anther of male flower is fertile and that of bisexual flower is sterile. In order to study male sterility-associated genes and their expressions and control, in this dissertation, the differences of anther's proteins between the bisexual and male flower of Xanthoceras sorbifolia Bunge. were analyzed by two-dimensional electrophoresis. And the distribution of special protein was studied by immunohistochemical localization.1. The anther's total proteins in the bisexual and male flower of Xanthoceras sorbifolia Bunge. from bud stage to florescence were analyzed by two-dimensional electrophoresis. The result showed: the proteins of two types of anther in bud stage had no difference, however there were at least 4 proteins (B1-4) expressed differently in florescence.2. The special protein B1 (49.9kD) was isolated, purified and gathered by preparative two-dimensional electrophoresis. And then it was used to immunize New-Zealand rabbit to prepare polyclonal antibody. The result of ELISA showed that the antibody titer reached 500. Western blotting assays showed that the antibody obtained was effective, specific and useful for further study. The immunohistochemical localization of B1 was carried out for the first time in two types of flowers of Xanthoceras sorbifolia Bunge.. The result showed that B1 distributed in tapetum. So it is speculated that B1 is an important protein related to the development of tapetum and male sterility.