Study Of Amino Acid Fluxs In Wether Duodenum Fed With SBM Diet And CSPM Diet

This research through 2 experiments to estimate rumen digesta overturn, the yield of rumen microbial crud protein(MCP) , total amino acid(TAA) discharge in duodenal , essential amino acid(EAA)discharge in duodenal ect, those nutrition index with comparison to make the measurement in sheep. Soybean meal (SBM) and cotton seed protein meal (CSPM) are the main protein nitrogen in the diet formula, designing four kinds of protein level as 11%, 13%, 15% and 17%, so there are 8 kind diets. I chose 4 growing sheep between 6 month to a half and 6 month about 30kg body weight (BW) as the experiment animals. Experiment data show, digesta discharge in duodenum fed with SBM diet are below respectively: 11.97 L/d, 11.49 L/d, 9.78 L/d,, 9.19 L/d; digesta discharge in duodenum fed with CSPM diet are: 8.33 L/ d, 6.75L/ d, 6.01 L/ d, 7.57L/d.It's clear that duodenum digesta discharge in sheep fed with SBM diet are over and above which fed CSPM diet. The discharge of the duodenal CP is also proof in sheep fed SBM diet. Duodenal TAA discharges are also proved higher in sheep fed SBM diet than fed CSPM diet, they are 646.19g/d, 510.61g/d, 617.10g/d, 572.93g/d; 284.55g/d, 235.63g/d, 291.57g/d, 270.01g/d respectively, EAA discharges are: 191.47g/d, 167.83g/d, 362.76g/d, 470.23g/d; 88.70g/d, 79.48g/d 173.26g/d, 216.47g/d respectively. They are also higher in sheep fed SBM than sheep fed CSPM. The yield of MCP in rumen are proved no difference between sheep those fed with SBM or fed with CSPM, they are(g/kg FOM): 21.37,24.71, 27.02,25.23;25.26, 27.37, 19.30, 14.43 respectively. But different CP level have to show the big influence about MCP yield in sheep fed CSPM.