Effects Of Exogenous Amino Acids On The Artificial Rumen Microbial Growth And Amino Acids Partner Of Ruminal Microorganism

In this study, four Xuhuai Goat wethers fitted with permanent ruminal cannulas were used to provide rumen liquor in vitro. The objectives of this study were to determine the effects of Met and BCAA on rumen fermentation and microbial growth. The removal method was introduced to study the effects of AA on rumen fermentation and the limitation of microbial growth. The ratio of EAA was carried out to study the effects of different amino acids profile patterns on ruminal fermentation parameters and rumen microbiology. The results of the study showed:1. The effects of Met and BCAA on rumen fermentation and microbial growth:The pH-value ranged between 6.16 and 6.56, and the highest mean value was observed in the group Met (6.38), and the lowest mean value was observed in the group Val (6.25); Concentration of NH3-N ranged between 6.04 to 10.87 mg/100ml, and the lowest mean value was observed in the group Val (7.33mg/100ml), and the highest mean value appeared in the group Leu (9.78mg/100ml). The highest of TVFA concentration was dropped in the group Leu (41.52mmol/L), and the lowest was observed in the blank group (36.01mmol/L). There was a significant difference in the each component VFA and the percentage of each component VFA's TVFA (P<0.05). The ratio of Acetic acid/Propionate was slightly higher than 3:1, group A was significantly higher than the group B (P <0.05), and the difference among the groups was not significant (P> 0.05). The maximum of parasite protein, bacterial protein and microbial protein was group Leu. Urea nitrogen has been reduced in each group with the time. The impact of the addition of met and BCAA on urea nitrogen was no significant (P> 0.05). There was no significant impact of training time and the addition of met and BCAA on GOT (P> 0.05). With the time extended, the activity of GPT increased. The addition of met and BCAA on GPT after 8h have differences. Ratio of protozoa and bacteria in group C up to 183.79%, and group B was a minimum of 137.71%. The highest of microbial AA-N ratio was group C, the lowest was group A, the group D of bacterial AA-N was highest, the lowest was group E, the highest of protozoa AA-N was group E, the lowest was group A. Mthionine and BCAA affect the AA composition of rumen micro-organisms, such as Glu, His, Arg, Ala, Pro, Ile (P <0.05), while the other amino acids were no significant differences (P> 0.05).2. The effects of AA on rumen fermentation and the limitation of microbial growth:The pH-value ranged between 6.01 and 6.45, and the highest mean value appeared in the group C (6.23). Concentration of NH3-N ranged between 7.98 to 29.05 mg/100ml, with the highest mean value occuered in the group A (18.16 mg/100ml). The highest of TVFA concentration was dropped in the group A (73.96mmol/L), and the lowest was observed in the group D (70.27mmol/L). There was a significant difference in the each component VFA and the percentage of each component VFA's TVFA (P<0.05). The ratio of Acetic acid/Propionate was close to 3:1 and the difference among the groups was not significant (P> 0.05). The order of the restricted role of AA-specific default on rumen micro-organisms (bacteria and protozoa) was that: BCAA> aromatic AA> methionine> lysine. The highest of P/B ratio was group B (101.37%), and the lowest was group D (88.22%). The highest of microbial AA-N ratio, bacteria AA-N, protozoa AA-N was group E, group D and group E, and the lowest of them was group C. Some amino acids concentration of rumen microbiology varied significantly (P<0.05), such as Ser, Ala, Cys, Leu and Lys, and other amino acids were no significant differences (P> 0.05). Microbial diversity was demonstrated in SSCP fingerprint clearly, revealed that the structure of bacteria or protozoa community was altered by substrates. There was a certain restrictive role of AA-specific default on the growth of R. amylophilus, B. fibrisolvens and P. ruminicola.3. The effects of different amino acids component patterns on ruminal fermentation parameters and rumen microbiology:The pH-value ranged between 6.12 and 6.57, and the lowest mean value was observed in the group C (6.25), and the highest mean value appeared in the group A (6.29). Concentration of NH3-N ranged between 3.45 to 30.07 mg/100ml, with the highest mean value occuered in the group D (12.88 mg/100ml). The highest of TVFA concentration was dropped in the group C, followed by the group D and the group B. The lowest was observed in the group A, comparing with the group B, C and D, the difference is notable (P<0.05). However, the difference among in the group B, C and D does not notable (P>0.05). The ratio of Acetic acid/Propionate was close to 3:1 and the difference among the groups was not significant (P> 0.05). There was a certain effect of different amino acids component patterns on rumen microbial growth. The order of microbial protein production may be out that: group B > group A>group C >group D. The difference of P/B value and AA-N among the groups was significant (P<0.05). Some amino acids concentration of rumen microbiology varied significantly (P<0.05), such as His, Pro, Leu, Phe. The genetic fingerprint analysis revealed that, microbial profile was modified by substrates within bacteria or protozoa community. The component patterns of Amino Acids can stimulate the growth of R. amylophilus, B. fibrisolvens and P. ruminicola with different influence.