The Incidence And The Preventive Measures Of Mastitis In Dairy Cattle At Different Feeding Condition

Mastitis was the most serious disease to dairy farming. In order to find out the relationshipbetween the mastitis occurrence rates and the different farm conditions, the SMT analysis tothe subclinical mastitis were carried out in a big dairy farm (A farm) at Yanchen area, northof Jiangsu during Jan of 2002 to Oct of 2003 .The contrast tests were also done hi differentfarm conditions of B, C, D, E, F, G, H farms. In this experiment, 2250 dairy cows and9666 udder regions were determined. The result showed: positive were 1425, the cattlepositive incidence was 55.9%; positive udder regions were 2677,the udder region positiveincidence was 27.7%.In A farm,2140 cattle were determined during 22 months, and positive catties were 1209,_the cattle positive incidence was 56.5%; positive udder regions were 2152,the udder regionpositive incidence was 26.6%. The statistic analysis were done according as differentseasons, and the result showed that the cattle positive incidence was no obvious relationwith seasons.The analysis results to 9666 udder regions showed that the mastitis occurrence rates werealmost same in left and right udder regions. And the mastitis occurrence rates between frontand back udder regions had obvious relation with the subclinical mastitis occurrence rates,the subclinical mastitis occurrence rates were obvious high in back udder regions.The subclinical mastitis analysis and statistic analysis were carried out in 220 catties of 6different feeding scale farms, 271 catties of 3 different milking method farms and 221catties of 2 different feeding nutrition farms. The results showed that the incidence rate ofsubclinical mastitis had no relation with different seasons, feeding scale, milking method,nutrition.Statistic analysis were done in 208 catties of 1 different feeding condition farm, 64 cattiesof different original diseases, 193 catties of different times of disinfection, 306 catties ofdifferent isolation of sick dairy cow and 210 catties of 3 different milking with rationalprogram farms. The results showed that the occurrence rate of the subclinic mastitis wasobviously connected with different feeding condition, original diseases, times ofdisinfection, isolation of sick dairy cow, milking with rational program.In order to find out the main mastitis pathogens in Yanchen and to take rational preventionand cure methods, the pathogens isolated and identified in milk from 16 dairy cow in 4dairy farms (including 7 clinical mastitis milk and 9 subclinical mastitis milk) were done in Sep of 2903. The shape and hemolysis of bacterium clones were observed after cultivating on rabbit blood agar culture medium and MacConkey medium. Single bacterium clone was picked out and inoculated in THE agar medium, after culturing, single clone was selected to inoculate in broth at 37 "C for 24 hours. Isolates were identified using conventional methods: Gram-stained cell morphology, colony morphology , micro-biochemical test and then stored at -20 *C . 16 strains were isolated from 13 milk samples (including various 2 strains in 3 individual milk samples ), no bacteria were isolated in 3 milk samples. The isolated strains contained 5 Staphylococcus aureus , 5 E. coll, 4 Streptococcus and 2 Yeast fungu, and this indicated that the pathogens in this area were almost same with other area's reports.