Research On Chemical Components Of Essential Volatile Oils From Cicuta Virosa L. And Its Repellent Effect On Microtus Brandti

Repellent effect is a way to harmonize the relationship between pests and environment. Exploiting the rodent repellents from plant materials has promising applied prospect.This work was based on the Cicuta virosa L., which were extracted by using steam distillation and analyzed with GC/MS (gas chromatography and mass selective system). Both the extraction by using ether and essential oils were compared and analyzed respectively by TLC (thin-layer chromatography) technique. The analyzing based on TLC, which the chromatographic solution was hexane to ethyl acetate amount to 3 to 1 and the thin-layer was GF254, showed that there were 7 sample spots to the ether extractions, 4 sample spots to the essential oils and the Rf value were 0.18, 0.29, 0.43 and 0.66, respectively. The analysis demonstrated that 40 main components were revealed with GC-MS and 32 of which were identified, up to 91.48 percent. The main components of the essential oils contained unsaturated alcohols. Cinnamaldehyde, a precursor of cinnamamide, which is an identified repellent, were shown in the essential oils. Identification of essential oils will provide a foundation for further utilization and development.Next, the poisonous characteristic and repellent effectiveness of essential oils were studied. The poisonous tests showed that Cicuta virosa L. has strong exciting effect on Brandt's voles' nerve center system. Upon Cicuta virosa L. treatment, Brandt's voles refused to eat, lost weight, and had impaired digest and liver. In no choice tests, the repellent effects of 50 percent and 15 percent Cicuta virosa L. were 67.60 percent and 41.92 percent to Brandt's voles, respectively. In choice test, the repellent force of 50 percent and 15 percent Cicuta virosa L. were 68.83 percent and 41.56 percent to Brandt's voles, respectively. Both of the tests showed high repellent effect. The molecular structure of repellent component is complex. Except Cinnamaldehyde, we don't know whether other molecules have contributions for repellent, This is something deserves further investigation.