Studies On Cow Mastitis

Studies included isolation and pharmacosensitive test of parthogen from clinical mastitis in cows, and treatment experiment in cows suffered from clinical mastitis. The herb disinfectant was prepared and the cow with subclinical mastitis was treated with it. Pathologic observation was maded for the teat tissure of cows with subclinical mastitis. Studies included 8 experiments majorly as follows: Test 1: The findings of mastitis in cowsClinical mastitis and subclinical mastitis in cows were investigated in Tai'an Jinan and Qingdao.The result showed that: the ratio of clinical mastitis in cows was 8.96%, 8.33% and 9.87% in 3 areas respectively. The incidence of subclinical mastitis was 55%, 4.48%, 34.48% and 44.83% in forward left, left back, forward right and right back udder region respectively; The incidence of subclinical mastitis was 75% and 64.7% in Jinan and Qingdao respectively, The incidence was 31.8%, 30.8%, 32.8% and 46.97% in each udder region spectively. As the raising of the number of foetustimes in cows, the incidence of subclinical mastitis had the tendency of rising. The incidence of the disease with 3 and 4 of foetuwas highest. Test 2: Isolation and biochemical test of clinical bacterium from cows with clinical mastitisStaphylococcu saureus, Reptagalactine, Salmonella, Citrobacter, Corynebacterium bovis, Escherichia coli and yeast bacteria were isolated from 43 udder region and milk from 17 cows with clinical mastitis. yeast bacterium took 30.23% at most in all isolated bacterium.Test 3: The pharmacosensitive test of bacterium from cows with clinical mastitis The result of pharmacosensitive test with medicine for the 5 kinds of bacterium which resulted in clinical mastitis showed that: Salmonella was sensitive to Amikacin, Gentamicin, enrofloxacin, Kanamycin, Cefoperazne, Cefuroximeaxetic, Cefazolin, Ceftriaxone, Ceftazidime, Cefotaxime; but not sensitive to penicillin, streptomycin, tetracycline, erythromycin, sulfamethoxazole, vancomycin, rifampin. Staphylococcus aureus was very sensitive to ampicillin, gentamicin, kanamycin, Cefoperazne, Cefuroximeaxetic, Cefazolin, Ceftriaxone, Ceftazidime, Cefotaxime, tobramycin, but not sensitive to penicillin, streptomycin, norfloxacin, sulfamethoxazole, et al. Citrobacter was very sensitive to gentomicin, amikacin, kanamycin, Cefoperazne, Cefuroximeaxetic, Cefazolin, Ceftriaxone, Ceftazidime, Cefotaxime, but not sensitive to penicillin, streptomycin, tetracycline, sulfamethoxazole. Streptococcuszooepidemicus was very sensitive to penicillin, Cefoperazne, Cefuroximeaxetic, Cefazolin, Ceftriaxone, Ceftazidime, Cefotaxime, but not sensitive to kanamycin, norfloxacin. Escherichia coli was sensitive to amikacin, Cefoperazne, Cefuroximeaxetic, Cefazolin, Ceftriaxone, ceftazidime, cefotaxime, Enrofloxacin; but not sensitive to penicillin, streptomycin, trythromycin and tetracycline. Test 4: The treatment experiment in cows with clinical mastitis Treatment experiment showed that: enrofloxacin and herb had the heighest clinical cure rate (83.33%, 100%); ampicillin, cefoperazone and long effective penicillin took the second place (66.7%, 75%, 70%); penicillin and streptomycin had the worst treatment effect (51.88%). Test 5: The pharmacosensitive test of 17 kinds of Chinese herbal medicine for 4 kinds of bacterium that resulted in mastitis in cowsThe test showed that: salmonella was sensitive to Fructus chebulae, Herba Houttuyniae, Herba Taraxacicum radice. Staphylococcus aureus was sensitive to Radix Seuteliariae, Fructus chebulae, Rhizoma Coptidis, Flos Carfhami and Herba Houttuyniae.Citrobacter was sensitive to Fructus Chebulae. Escherichia coli was sensitive to Radix Seuteliariaecterium, Herba Houttuyniae, Herba Taraxacicum Radice and Rhizoma Coptidis. Test 6: The preparation of the herb neat disinfectant for ow nippleThe 4 mixture of traditional Chinese medicine were made of herb that restrained pathogens well. The exp...