| Soybean is abundant in protein but deficient in amino acids containing sulfur, which reduce the nutrition value of soybean storage protein and restrict the absorption and use of soybean storage protein inside human body. It is possible that variety of high 11S globulin or high 11S/7S can be bred by one or more breeding way. The experiment analyzed the differences of subunits type among varieties and analyzed the contents of subunits of soybean storage protein by SDS-PAGE. The experiment materials consist of both 377 varieties overseas and inland and 5 varieties cultivated 11 locations including Heilongjiang province, Jilin province and Liaoning province. The main research results are as follows:1. The band types of G. Max by SDS-PAGE are same approximately, each variety have 20 bands or more. The main subunits consists of a, a,r,b,A3, Acid, basic and A5,the molecule weight of these subunits is 77.45, 66.72, 60.44, 47.60, 40.71, 35.96, 20.88 and 13.97 KDa respectively. The p subunit of 7S globulin of G Max has double-band type; the acid and basic subunits have 6 and 4 or more bands. This experiment obtained 1 variety which contained double-band in P globulin and 3 varieties lacking one subunit of acid and one subunit of basic and A5 subunit.2. The contents of different subunits or the same subunit of different varieties vary greatly. The average contents of a, a,r,b, and total 7S globulin are 6.58%, 7.30%, 4.66%, 6.07% and 29.86% respectively, the range of those subunits are 6.75%, 9.46%, 7.85%, 8.64%, 19.15% respectively, and the variable efficient of those subunits are 20.91, 20.54, 28.74, 22.92,12.88 respectively; The average contents of A3, A5, acidic, basic and total US globulin are 4.6%, 2.36%, 28.49%, 21.97%, 58.26% respectively, the range of those subunits are 6.57 %, 5.96%, 16.95%, 22.52%, 29.1% respectively, and the variable efficient of those subunits are 20.3, 42.78, 19.12, 15.25,9.94 respectively.3. The variance of 7S globulin of locations and varieties and years and the interaction between location and varieties and interaction between variety and year is significant at 0.05 level respectively; The variance of US globulin of locations and varieties and years and interaction between variety and year is significant at 0.05 level respectively; Negative correlation significantly appears between US globulin and 7S globulin; The max and min value of 11S/7S of globulin is 3.70 and 1.34 respectively, the average is 2.36. The variance of varieties and locations and years and the interaction between year and location of 11S/7S is significant, other interactions is not significant; The variation among locations is high than that among varieties in 11S/7S, and the variation of varieties of 11S/7S overseas is high than that inland. All these show that varieties of high 11S globulin or high 11S/7S can be bred.4. The 11S/7S value of 5 locations in Heilongjiang Province increase with the increase of longitude.5. The type of bands of SDS-PAGE image among G. Max and G soja and big seed soybean (>22g) and little seed soybean (< 14g)and green soybean and brown soybean is similar.Candidate: Jiang ZhenfengSpecialty: Crop Genetics and BreedingSupervisor: Prof. [Yang Qingkai]... |
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