Molecular Marker Of Dominant Glandless Gene Gl₂～e In G.hirsutum L.

Glandless or gossypol-free cotton is of great value to develop the utilization of cottonseeds and cotton scientists have been interested in developing more applicable glandless breed.It takes 8-10 years to breed a new cultivar . Molecular markers can be used as a selecting tool in the breeding because the molecular marker-assisted selection (MAS) can not only precisely select the targeted gene but also quickly delete linked drags. It can eliminate time by the MAS and enhance the efficiency.The objectives of the study are screening the markers linked to the dominant Glandless gene Gl2e The markers can be applied in marker-assisted selection breeding. The main results are as follows:(1) In the study, the Near-isogenic lines (NILs) CRI12 and Glandless CRI 12 are used as two parents. There are 161 Glandless and 48 Gland plants in the F2 population .The segregation ratio of : Gland agree with expectation 3:1. It shows the Glandless gene G12e was controlled by a dominant gene.(2) NILs was adapted to identify RAPD markers linked to the Glandless gene G12e . DNA bands are amplified from 380 random 10-mer primers. Only one polymorphic band OPD06.1530 was found in the glandless NIL but not in the other .(3) The polymorphic fragment was cloned and sequenced. Nucleotide sequence information was used to design 18-20-mer oligo nucleotide primer for PCR amplification.(4) The NILS and its F2 population ware screened using SCAR primer. And the SCAR marker (SCOPD06.1530) generated from the primer showed the pattern of segregation as same as the original RAPD marker(4) The map distance between G12e and marker-OPD06.1530 is 6.4cM...