RAPD Analysis Of Germplasm Resources On Peach

Peach, originating from the western of China,has developed many species and categories in the long period of natural selecting and artifical domestication.Lots of work have been done in morphology,pollen and isoenzyme with some performance about the identification,taxonomy and evolution of peach.But,thereare still some divergence in result and limitation in technology.By molecular biological methods,genetic relationship in resources of peach germplasm was studied.The results are as follow:(1) Based on the analysis of six isolation methods for genomic DNA of peach, the results showed that the step by step centrifugation method is optimal for isolation method.(2) Genomic DNA of fruits tender leaves flower bud anmal bast biennial bast of peach was isolated by the step bystep centrifugation method,the result shows yong leaves and fruits were optimal for isolation of genomic DNA of peach.(3) Eighteen decamer arbitrary primers, which had polymorphisms and clear bands, were selected out of two hundred twenty; the primers were successfully applified in analysis of peach germplasm.(4) 110 peach species and categoryies were studied by selected 18 primers and 88 divercification seats. All the materials were taxonomied into 5 typies at 17 distance: maoyingtao guanghetao shantao gansutao putongtao.Xinjiangtao was claffied with scientific name as Army gdalus persica var. Ferganensis.The result revealed: guanghetao lies in the primitive position. Molecular phylogeny of species in peach genus by DNA fringerprinting was as follows: guanghetao-gansutao-shantao-putongtao(xinjinangtao). Guanshangtao being a cultivar are similar to xinjiangtao in evolution status.(5) Putong peach had higher genetic similarity and frequency of gene exchange by dendrogram of RAPD bands. According to places of the accessions in the WARD cluster, putong peach could be divided into crisp groupmitao grouphoney peach group pantao group nectarine group,the yellow peach group and guanshang peach group.