The Research Of Exogenous DNA Transformation Of Wheat Via Ion Beam

To meet the need of the quality breeding aim of our laboratory, to establish an effective selecting system on the basis of protein is the most important thing. According to the tested wheat sample supported by the china corn quality supervision &test center, the sample spectrums were selected, through mathematic management; a calibration model and an equation were founded accordingly. The new calibration was tested by another group of sample and the result was very good, then the Infrared Reflectance Spectroscopy instrument set up a new accurate, fleet and intact system for wheat protein test. After some contrast experiment, the result by NIRS is inline with the chemical one. In about two years, about 80,000 wheat samples from genetic transformation had been tested, and the system played an important role in the wheat quality breeding.In the light of the good wheat breeds and series of our country, the transformation time and the efficiency of different wheat breeds and different soy and different legume were studied respectively. Result shows that the finest transformation time is 48 hour. The transformation efficiency is different among different wheat breeds when the exogenous DNA is certain. And the efficiency is different also among different exogenous DNA when the wheat breed is determined. The efficiency of soy DNA transformation is the most stable and better one among different legume. In the last two years, we have got 133 plants, whose protein content is higher than 18%, and 30 plants higher than 19% in Tl. The number of samples in Tl whose protein content distinguished dramatically from CK ranges from 5 to 20 per. The high protein samples were raised the next year; we can see that the trait of high protein can be inherited in T2.Generally speaking, form the results of different area and of different wheat breeds, it shows that the method of exogenous DNA transformation of wheat via ion beam is indeed very potent; it cannot only increase the protein content of wheat, but also contribute to the improvement of the agricultural properties. For the moment, the mechanism of the method is not very clear, but it may be a nonsexual and distant related breeding way. Because of its simplicity, safety, and no need of cloning gene, it may be an effective way of broadening the wheat breeds and its genetic diversities, and thus it will have a bright future in quality breeding.