| In 1999,in order to study Nitrogen ion beam mutation effect, 3000 air-dried seeds of each variety(line) Yumai 52, Yunong 118, Yumai 2,and Yuinong 92349 were implanted by Nitrogen Ion Beam, mutation dose is 1-6(X 1017N+/cm2). Non-treated seeds were control. These seeds were divided into two parts: one part was planted agricultural fields, another was conducted germination test and potted plant test. At the same time, we selected Yumai52 as gene transfer receipt, soybean lines 86-4 and 88-48 as gene transformation servant, aiming at the study of Ion beam induced Soybean DNA transferred into wheat in 1999. In 2000, wheat varieties (lines) Yumai54, Yumai52, Yuinong 92349andYumai 49, 4 and, and soybean varieties (lines) Yudoul2, 22,24 and 89013 were selected. Two years wheat transfer receipts were implanted by Nitrogen Ion Beam, mutation dose is 2X1017N~Vcm2 .Nitrogen Ion Beam implantation process was conducted by Ion Beam laboratory of Zhengzhou University. During the two periods, Nitrogen Ion Beam's parameters, energy and the Velocity of flow, are 30KeV, 30 Milliampere (MA), individually. Fields tests were conducted in wheat breeding fields of Zhengzhou science-teaching farm of Henan Agriculture University in 1999-2001.During dada analysis, statistics methods were used, such as analysis of variance, correlation analysis, variance homogeneity test, x 2-test and t-test.To Nitrogen Ion Beam mutation biological effects, the results showed that: 1. The effects of Ion implantation to variety germination are different. That is, the difference of germination rate isn't significant, however, following the dose increase, germination potential decrease. 2. To young wheat plant characters, which are seedling height, first leaf length, number of seminal roots and number of second-class roots, which tendency of ion implant effects is same: first increase, then decrease, and then increase, finally decrease. Moreover, there is variety difference to dose variance in second-class seminal roots, in addition to this character, the dose variance in other three characters is significant. 3.To mature wheat plant characters, which are plant height, spike length, number of spike, spikelets per spike, non-spikelets per spike, grain weight per plant and 1000-grain weight, the variance between varieties and doses, as well as their interaction, are significant. Following dose increase, some characters tendency is first increase then decrease and then increase, such as plant height, number of spike, spikelets per spike, grain weight per plant; others' are first decrease then increase and then decrease. There-58-are differences between varieties and characters in the same variety.As far as Soybean's DNA transferred into common wheat induced by Ion beam is concerned, the transferring effect of first generation and its genetic characters of second generation were studied, and the effect of two main factors, servant and receipt, on transfer rate individually was discussed. The results are as follows, 1 .Wheat quality was affected by Ion beam, the affecting extent of varieties is different, but in the same variety, Ion implant effect had no difference among three quality characters, which are protein content, gluten content and absorption ration. Compared with control, transferred wheat quality has no difference on the whole, however, its variance extent was increased and some plants of high protein content were found. 2. And this high protein content character was inherited in the second generation. There is significant positive correlation between transfer rate of the second generation and protein content of the first generation. That is, the higher protein contents the higher transfer rate. 3.The difference of transfer rate was significant between different servants of the same receipt and different receipts of the same servant. At the same time, this difference was also found at second generation. Based on the results, we selected three good receipt-servant combinations, which areYunong92349/Yudou22, Yumai49/8903land Yumai2/Yud... |
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