| In this research, the chalcone sythase (CHS) gene was isolated from Lilium hybrida Sorbonne, which played an essential role in pigmentation and biosynthesis of anthocyanidins in the early developemental stage of flowers. The bioinformatics and functional analysis of CHS gene was carried out in order to give theoretical basis and technical support for cultivation of novel lily with blue colour. The results of.this research1,Isolation CHS gene from Lilium hybrida SorbonneThe cDNA sequence of CHS gene were isolated from Lilium hybrida Sorbonne using the degenerate primers and Reverse Transcription PCR technology. The sequence was submmitted to Genbank(Accession No:GQ483376) and named as LCHS2.2,Vector construction of over-expression and RNA interference with LCHS2 gene from Lilium hybrida SorbonneVectors of over-expression and RNA interference of LCHS2 gene from Lilium hybrida Sorbonne drived by constitutive pattern CaMV35S promoter and special pattern PCHS promoter were constructed, respectively.3,Transient expression analysis of LCHS2 gene from Lilium hybrida Sorbonne The detached leaves of petunia hybrida were transformed by Agrobacterium-mediated vacuuminfiltration. The transcription of LCHS2 gene from Lilium hybrida Sorbonne and protein expression of GFP and GUS gene were detected to give an early inspection of target gene for genetic transformation.4,Transformation of petunia hybrida by VIGS systemTransformation of petunia hybrida by VIGS system was carried out. The phenotypes of gene silencing(change of flower pigmentation)appeared with comparison to wild plants. The phenotypes could be divided into chimeric pattern, mosaic pattern and mediate pattern. The anthocyanidin content of flowers of these patterns decreased to varying degrees. |
