Study On Transferring ACC Deaminase Gene Into Hami Melon(Cucumis Melo L.) Of Xinjiang

ACC deaminase can catalyzes metabolism of ACC to ammonoa and α-ketobutyrate . Removal of ACC in the transformed plants reduces ethylene synthesis and delays fruit ripening. The transfer of a gene,which encodes 1-aminocyclopropane-1- carboxylic acid deaminase (ACCd), into Hami melon(Cucumis melo.L) of xinjiang (Huanghou and Jiashigua) by Agrobacterium tumefaciens -mediated method was studied. The kanamycin -resistant transgenic plants were regenerated after cotyledon explants of 5-day-old were infected 5-8 min in Agrobacterium tumefaciens, co-cultured 2 days, and then by using debacterium, differentiation and root culture. The frequency of bud differentiation can be increased with AS and AgNO3 in culture medium. The experiments of transplanting showed that the survival rate of plants by trained and water-cultured method was higher and the growth of later stage was faster . The rooting and growing rates of transgenic plants were less than that of controlled plants. Transgenic plants were confirmed by kanamycin-resistant experiment and PCR analysis. The results indicated that the 1kb ACC deaminase gene was integrated into Hami melon genome. Peroxide isozyme analysis showed that the bands of transgenic plants were more than those of controlled plants, which may be caused by the transfer of ACC deaminase gene into Hami melon plants. Ethylene measurement preliminarily proved that transgenic plants can partly inhibit ethylene synthesis.