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Transcriptomics And Proteomics Of Hami Melon Responding To Penicillium Infection And Change Analysis Of Resistance-related Enzymes

Posted on:2016-03-01Degree:DoctorType:Dissertation
Country:ChinaCandidate:C H DanFull Text:PDF
GTID:1223330464465536Subject:Food Science
Abstract/Summary:PDF Full Text Request
Hami melon is the characteristic fruit resources in Xinjiang. In the preservation and transportation process of postharvest, the diseases infection has become the bottleneck issure which restricts the industral development of Hami melon. According to statistics, the total production of Xinjiang Hami melon was 2.4 million tons in 2014, while the rate of decay reached to about 30% in the preservation. The preliminary research showed that the infection of Penicillium was the inducing of the decay of fruits. The research of response reaction mechanism of Hami melon to Penicillium infection is rarely reported. That has become the key scientific concern for the decay control of Hami melon. In this study, the types, functions and number of differentially expressed genes of the fruits of Hami melon in the infection of Penicillium were analyzed through the technology of transcriptome and proteome, and the important differential proteins related to the course of the disease were identified. The response reaction mechanism of Hami melon to Penicillium infection was revealed, which would promote the regulation of related resistance genes and proteins. The specific results and conclusions are as follows.1. 50502 Unigenes were obtained through transcriptome analysis, 71.28% of which could be annotated to Non-redundant(NR) database. Through the Pathway analysis, 49% of the Unigenes could be mapped to 128 metabolic pathways; 35080 CDS were obtained compared with the protein libraries, 1395 CDS were obtained by ESTscan prediction; In this study, 10526 Unigenes were unannotated, which probably were the novel transcription, or the genetic traits of Hami melon fruit differentiating from other species. The mining of novel transcription or specific genes of Hami melon differentiating from other species could be realized through multivariate validation.2. In the different stages of Penicillium infection, the gene expression of Hami melon fruit was significantly different. Comparing uninfected samples with 48 hours infected ones, there were 15089 differentially expressed genes. Comparing with 60 hours infected ones, there were 15089. In the 48 hours infection of Penicillium, the expression of related genes, such as superoxide dismutase(SOD), β-glucosidase 24, peroxidase(POD) N1, were rise to defense the infection. In the 60 hours infection, the expression of 8 related genes, such as leucine riched repeated protein, POD5, calcium dependent protein kinase, were rise to defense the infection. In the whole infection, the genes of chitinase I, POD73, glutathione s-transferase were continuely rise. The result showed that response reactions of Hami melon to Penicillium infection could be regulated by the expression of key genes in the pathways, such as glutathione metabolism, phenyl-propane biosynthesis, phenylalanine metabolism, flavonoids synthesis, metabolism of glutamic acid and methionine, br sterol(BRs), salicylic acid signal3. There were two different of immune system in harvested Hami melon fruits which could induce defense reaction. One was PAMP-triggered immunity, which produced the allergic reactions through regulating the expression of the genes such as the calcium dependent protein kinase, respiratory burst oxidase, leucine repeated receptor protein kinase, calcium regulatory proteins, and rise the expression through regulating the disease-course inducing related proteins(PRI) such as Tu extend factor, cell mitogen activated protein kinase 1; Another was Effector-triggered immunity, which induced the allergic reactions through regulating the expression of the genes such as activation disease-resistant protein(RPM1), disease-resistant protein(RPS2), and improve the disease resistance of Penicillium through regulating the disease-course inducing related proteins such as transcription factor(MYC2) and jasmonic acid ZIM domain contains protein(JAZ).4. Through the identification of fruits proteins of Hami melon before and after being infected by Penicillium using the isotope labeling technology of relative and absolute quantitative(i TRAQ), 4855 expression protein of the fruits were obtained, including 76 function-unknown protein. In the different stages of Penicillium infection, identified differential proteins were different. Comparing uninfected samples with 48 hours infected ones, there were 179 differential proteins; Comparing with 60 hours infected ones, there were 413; After further analysis, 25 common differential proteins were selected, such as cut inside chitinase(MCHT-2), peroxidase 52, which continuely played the role of resistance in the 60 hours infection; and have; and such as fruit protein, glutathione S transferase(PARB), cut inside acidic chitinase, etc., which performed the important role of resistance in the 48 hours infection;5. Correlation analysis between differential proteins and differentially expressed genes showed there were 107 associated differential proteins in the group of uninfected samples versus 48 hours infected ones(0-Vs-48), including 70 positive correlation differential proteins; and there were 253 associated differential proteins in the group of uninfected v.s. 60 hours infected(0-Vs-60), including 172 positive correlation differential proteins, and the largest proportion of differential proteins was art related protein. Through a STRING online analysis, the functions of three unknown differential proteins were predicted. Among them, Unigene 11705_QHMG probably possessed the response function to biological stimulation and defense; Unigene 11707_QHMG might involve in the defense response through regulating br steroids signal; CL3109. Contig1_QHMG performed the defense response function by regulating the synthesis of lignin and flavonoids.6. The resistance enzymes of Hami melon fruit, such as SOD, POD, CAT, β-1,3 glucanase, CHT, involved in the reaction against Penicillium infection; In the early storage, low temperature and fresh-keeping agent could induce the raise of activities of the resistance enzymes to remove free radicals; In the late storage, the infection of mold induced the increase of the activities of defense enzymes, which improved the disease resistance of the fruit.
Keywords/Search Tags:Hami melon, transcriptome, proteomics, differentially expressed gene, differential protein
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